Structure, Function and Regulation of CSB: a Multi-talented Gymnast

Overview

Journal Mech Ageing Dev

Specialty Geriatrics

Date 2013 Feb 21

PMID 23422418

Citations 36

Authors

Robert J Lake

Hua-Ying Fan

Affiliations

Soon will be listed here.

Abstract

The Cockayne syndrome complementation group B protein, CSB, plays pivotal roles in transcription regulation and DNA repair. CSB belongs to the SNF2/SWI2 ATP-dependent chromatin remodeling protein family, and studies from many laboratories have revealed that CSB has multiple activities and modes of regulation. To understand the underlying mechanisms of Cockayne syndrome, it is necessary to understand how the biochemical activities of CSB are used to carry out its biological functions. In this review, we summarize our current knowledge of the structure, function and regulation of CSB, and discuss how these properties can impact the biological functions of this chromatin remodeler.

Citing Articles

ATP-Dependent Chromatin Remodeler CSB Couples DNA Repair Pathways to Transcription with Implications for Cockayne Syndrome and Cancer Therapy.

Bilkis R, Lake R, Fan H Cells. 2025; 14(4).

PMID: 39996712 PMC: 11852979. DOI: 10.3390/cells14040239.

Perspectives in the investigation of Cockayne syndrome group B neurological disease: the utility of patient-derived brain organoid models.

Wang X, Zheng R, Dukhinova M, Wang L, Shen Y, Lin Z J Zhejiang Univ Sci B. 2024; 25(10):878-889.

PMID: 39420523 PMC: 11494160. DOI: 10.1631/jzus.B2300712.

Heat shock protein DNAJA2 regulates transcription-coupled repair by triggering CSB degradation via chaperone-mediated autophagy.

Huang Y, Gu L, Li G Cell Discov. 2023; 9(1):107.

PMID: 37907457 PMC: 10618452. DOI: 10.1038/s41421-023-00601-8.

The CSB chromatin remodeler regulates PARP1- and PARP2-mediated single-strand break repair at actively transcribed DNA regions.

Bilkis R, Lake R, Cooper K, Tomkinson A, Fan H Nucleic Acids Res. 2023; 51(14):7342-7356.

PMID: 37326017 PMC: 10415129. DOI: 10.1093/nar/gkad515.

Heterogeneous clinical features in Cockayne syndrome patients and siblings carrying the same CSA mutations.

Chikhaoui A, Kraoua I, Calmels N, Bouchoucha S, Obringer C, Zayoud K Orphanet J Rare Dis. 2022; 17(1):121.

PMID: 35248096 PMC: 8898519. DOI: 10.1186/s13023-022-02257-1.

References

Yu L, Zhu Z, Chan K, Issaq H, Dimitrov D, Veenstra T . Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra. J Proteome Res. 2007; 6(11):4150-62. DOI: 10.1021/pr070152u. View

Clapier C, Cairns B . The biology of chromatin remodeling complexes. Annu Rev Biochem. 2009; 78:273-304. DOI: 10.1146/annurev.biochem.77.062706.153223. View

Wollmann P, Cui S, Viswanathan R, Berninghausen O, Wells M, Moldt M . Structure and mechanism of the Swi2/Snf2 remodeller Mot1 in complex with its substrate TBP. Nature. 2011; 475(7356):403-7. PMC: 3276066. DOI: 10.1038/nature10215. View

Zhang Z, Fan H, Goldman J, Kingston R . Homology-driven chromatin remodeling by human RAD54. Nat Struct Mol Biol. 2007; 14(5):397-405. DOI: 10.1038/nsmb1223. View

Gelbart M, Bachman N, Delrow J, Boeke J, Tsukiyama T . Genome-wide identification of Isw2 chromatin-remodeling targets by localization of a catalytically inactive mutant. Genes Dev. 2005; 19(8):942-54. PMC: 1080133. DOI: 10.1101/gad.1298905. View

He X, Fan H, Narlikar G, Kingston R . Human ACF1 alters the remodeling strategy of SNF2h. J Biol Chem. 2006; 281(39):28636-47. DOI: 10.1074/jbc.M603008200. View

Newman J, Bailey A, Fan H, Pavelitz T, Weiner A . An abundant evolutionarily conserved CSB-PiggyBac fusion protein expressed in Cockayne syndrome. PLoS Genet. 2008; 4(3):e1000031. PMC: 2268245. DOI: 10.1371/journal.pgen.1000031. View

Gray L, Fong K, Pavelitz T, Weiner A . Tethering of the conserved piggyBac transposase fusion protein CSB-PGBD3 to chromosomal AP-1 proteins regulates expression of nearby genes in humans. PLoS Genet. 2012; 8(9):e1002972. PMC: 3459987. DOI: 10.1371/journal.pgen.1002972. View

Berquist B, Wilson 3rd D . Nucleic acid binding activity of human Cockayne syndrome B protein and identification of Ca(2+) as a novel metal cofactor. J Mol Biol. 2009; 391(5):820-32. PMC: 2728148. DOI: 10.1016/j.jmb.2009.06.078. View

10.

Fairman-Williams M, Guenther U, Jankowsky E . SF1 and SF2 helicases: family matters. Curr Opin Struct Biol. 2010; 20(3):313-24. PMC: 2916977. DOI: 10.1016/j.sbi.2010.03.011. View

11.

Racki L, Yang J, Naber N, Partensky P, Acevedo A, Purcell T . The chromatin remodeller ACF acts as a dimeric motor to space nucleosomes. Nature. 2009; 462(7276):1016-21. PMC: 2869534. DOI: 10.1038/nature08621. View

12.

Selby C, Sancar A . Human transcription-repair coupling factor CSB/ERCC6 is a DNA-stimulated ATPase but is not a helicase and does not disrupt the ternary transcription complex of stalled RNA polymerase II. J Biol Chem. 1997; 272(3):1885-90. DOI: 10.1074/jbc.272.3.1885. View

13.

Wu Y, Brosh Jr R . Distinct roles of RECQ1 in the maintenance of genomic stability. DNA Repair (Amst). 2010; 9(3):315-24. PMC: 2827648. DOI: 10.1016/j.dnarep.2009.12.010. View

14.

Yu A, Bailey A, Weiner A . Metaphase fragility of the human RNU1 and RNU2 loci is induced by actinomycin D through a p53-dependent pathway. Hum Mol Genet. 1998; 7(4):609-17. DOI: 10.1093/hmg/7.4.609. View

15.

Berquist B, Canugovi C, Sykora P, Wilson 3rd D, Bohr V . Human Cockayne syndrome B protein reciprocally communicates with mitochondrial proteins and promotes transcriptional elongation. Nucleic Acids Res. 2012; 40(17):8392-405. PMC: 3458532. DOI: 10.1093/nar/gks565. View

16.

Wu J, Lessard J, Olave I, Qiu Z, Ghosh A, Graef I . Regulation of dendritic development by neuron-specific chromatin remodeling complexes. Neuron. 2007; 56(1):94-108. DOI: 10.1016/j.neuron.2007.08.021. View

17.

Citterio E, Rademakers S, van der Horst G, van Gool A, Hoeijmakers J, Vermeulen W . Biochemical and biological characterization of wild-type and ATPase-deficient Cockayne syndrome B repair protein. J Biol Chem. 1998; 273(19):11844-51. DOI: 10.1074/jbc.273.19.11844. View

18.

Kyng K, May A, Brosh Jr R, Cheng W, Chen C, Becker K . The transcriptional response after oxidative stress is defective in Cockayne syndrome group B cells. Oncogene. 2003; 22(8):1135-49. DOI: 10.1038/sj.onc.1206187. View

19.

Wang X, Yeh H, Schaeffer L, Roy R, Moncollin V, Egly J . p53 modulation of TFIIH-associated nucleotide excision repair activity. Nat Genet. 1995; 10(2):188-95. DOI: 10.1038/ng0695-188. View

20.

Matsuoka S, Ballif B, Smogorzewska A, McDonald 3rd E, Hurov K, Luo J . ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science. 2007; 316(5828):1160-6. DOI: 10.1126/science.1140321. View