Mouse Hematopoietic Stem Cell Transplantation

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2013 Feb 13

PMID 23400432

Citations 9

Authors

Hui Cheng

Paulina H Liang

Tao Cheng

Affiliations

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Abstract

Hematopoietic stem cells (HSCs) are capable of self-renewal and multi-lineage reconstitution of hematopoiesis in irradiated transplant recipient mice. As such, bone marrow transplantation (BMT) is a major assay commonly used to examine murine HSC activity. BMT traditionally involves injection of HSCs into lethally irradiated recipients via the tail vein, then subsequently analyzing donor engraftment. Here, we describe the methods for assaying HSC reconstitution in direct, competitive, and serial BMT.

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