Analysis of Euglena Gracilis Chloroplast Deoxyribonucleic Acid with a Restriction Endonuclease, EcoRI

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1977 May 1

PMID 233724

Citations 4

Authors

J R Mielenz

J J Milner

C L Hershberger

Affiliations

Soon will be listed here.

Abstract

Cleavage of chloroplast deoxyribonucleic acid (DNA) of Euglena gracilis Z with restriction endonuclease RI from Escherichia coli (EcoRI) yielded 23 bands upon electrophoresis in gels of agarose. Four of the bands contained twice the stoichiometric amount of DNA. One of these bands contained two similarly sized fragments. The sum of the molecular weight of the 24 different fragments equaled the molecular weight of the circular molecule. The restriction fragments had different buoyant densities, with four having distinctly heavy densities in CsCl. Restriction fragments with a high buoyant density were preferentially lost when broken chloroplast DNA was purified by equilibrium density gradient centrifugation. Hybridization of chloroplast ribosomal ribonucleic acid to intact chloroplast DNA determined that there are two cistrons for 16S and 23S ribosomal ribonucleic acid. These two cistrons are located on six restriction fragments, all of which have buoyant densities greater than the intact molecule of chloroplast DNA.

Citing Articles

Intraspecific variation in the structural organization and redundancy of chloroplast ribosomal DNA cistrons in Euglena gracilis.

Wurtz E, Buetow D Curr Genet. 2013; 3(3):181-7.

PMID: 24190130 DOI: 10.1007/BF00429820.

Euglena gracilis Chloroplast DNA Codes for Polyadenylated RNA.

Milner J, Hershberger C Plant Physiol. 1979; 64(5):818-21.

PMID: 16661061 PMC: 543370. DOI: 10.1104/pp.64.5.818.

Inhibition of deoxyribonuclease activity in the medium surrounding plant protoplasts.

Slavik N, Widholm J Plant Physiol. 1978; 62(2):272-5.

PMID: 16660499 PMC: 1092103. DOI: 10.1104/pp.62.2.272.

Turnover-synthesis of chloroplast DNA in developing chloroplasts.

Walfield A, Hershberger C J Bacteriol. 1978; 133(3):1437-43.

PMID: 417068 PMC: 222182. DOI: 10.1128/jb.133.3.1437-1443.1978.

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