Systematic Functional Comparative Analysis of Four Single-stranded DNA-binding Proteins and Their Affection on Viral RNA Metabolism

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Feb 1

PMID 23365690

Citations 9

Authors

Haiyan Shi

Yonghui Zhang

Guohui Zhang

Jinlei Guo

Xun Zhang

Haiyan Song

Jianxin Lv

Jimin Gao

Yuepeng Wang

Litian Chen

Yue Wang

Affiliations

Soon will be listed here.

Abstract

The accumulation of single-stranded DNA-binding (SSB) proteins is essential for organisms and has various applications. However, no study has simultaneously and systematically compared the characteristics of SSB proteins. In addition, SSB proteins may bind RNA and play an unknown biological role in RNA metabolism. Here, we expressed a novel species of SSB protein derived from Thermococcus kodakarensis KOD1 (KOD), as well as SSB proteins from Thermus thermophilus (TTH), Escherichia coli, and Sulfolobus Solfataricus P2 (SSOB), abbreviated kod, tth, bl21, and ssob, respectively. These SSB proteins could bind ssDNA and viral RNA. bl21 resisted heat treatment for more than 9 h, Ssob and kod could withstand 95°C for 10 h and retain its ssDNA- and RNA-binding ability. Four SSB proteins promoted the specificity of the DNA polymerase in PCR-based 5- and 9-kb genome fragment amplification. kod also increased the amplification of a 13-kb PCR product, and SSB protein-bound RNA resisted Benzonase digestion. The SSB proteins could also enter the host cell bound to RNA, which resulted in modulation of viral RNA metabolism, particularly ssob and bl21.

Citing Articles

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