Sorting Short Fragments of Single-stranded DNA with an Evolving Electric Double Layer

Overview

Journal J Phys Chem B

Publisher American Chemical Society

Specialty Chemistry

Date 2013 Jan 30

PMID 23356906

Citations 1

Authors

Jiamin Wu

Shuang-Liang Zhao

Lizeng Gao

Jianzhong Wu

Di Gao

Affiliations

Soon will be listed here.

Abstract

We demonstrate a new procedure for separation of single-stranded DNA (ssDNA) fragments that are anchored to the surface of a gold electrode by end hybridization. The new separation procedure takes advantage of the strong yet evolving nonuniform electric field near the gold surface in contact with a buffer solution gradually being diluted with deionized water. Separation of short ssDNA fragments is demonstrated by monitoring the DNA at the gold surface with in situ fluorescence measurement. The experimental results can be rationalized with a simple theoretical model of electric double layer that relates the strength of the surface pulling force to the ionic concentration of the changing buffer solution.

Citing Articles

Melting dynamics of short dsDNA chains in saline solutions.

He Y, Shang Y, Liu Y, Zhao S, Liu H Springerplus. 2015; 4:777.

PMID: 26697287 PMC: 4679706. DOI: 10.1186/s40064-015-1581-7.

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