Platform for in Situ Real-time Measurement of Protein-induced Conformational Changes of DNA

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2010 Jan 19

PMID 20080702

Citations 4

Authors

Philipp S Spuhler

Jelena Knezevic

Ayca Yalcin

Qiuye Bao

Erika Pringsheim

Peter Droge

Ulrich Rant

M Selim Unlu

Affiliations

Soon will be listed here.

Abstract

A platform for in situ and real-time measurement of protein-induced conformational changes in dsDNA is presented. We combine electrical orientation of surface-bound dsDNA probes with an optical technique to measure the kinetics of DNA conformational changes. The sequence-specific Escherichia coli integration host factor is utilized to demonstrate protein-induced bending upon binding of integration host factor to dsDNA probes. The effects of probe surface density on binding/bending kinetics are investigated. The platform can accommodate individual spots of microarrayed dsDNA on individually controlled, lithographically designed electrodes, making it amenable for use as a high throughput assay.

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