Enterovirus 71 Infection Cleaves a Negative Regulator for Viral Internal Ribosomal Entry Site-driven Translation

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2013 Jan 25

PMID 23345520

Citations 25

Authors

Li-Lien Chen

Yu-An Kung

Kuo-Feng Weng

Jing-Yi Lin

Jim-Tong Horng

Shin-Ru Shih

Affiliations

Soon will be listed here.

Abstract

Far-upstream element-binding protein 2 (FBP2) is an internal ribosomal entry site (IRES) trans-acting factor (ITAF) that negatively regulates enterovirus 71 (EV71) translation. This study shows that EV71 infection cleaved FBP2. Live EV71 and the EV71 replicon (but not UV-inactivated virus particles) induced FBP2 cleavage, suggesting that viral replication results in FBP2 cleavage. The results also showed that virus-induced proteasome, autophagy, and caspase activity co-contribute to EV71-induced FBP2 cleavage. Using FLAG-fused FBP2, we mapped the potential cleavage fragments of FBP2 in infected cells. We also found that FBP2 altered its function when its carboxyl terminus was cleaved. This study presents a mechanism for virus-induced cellular events to cleave a negative regulator for viral IRES-driven translation.

Citing Articles

Insight into the Life Cycle of Enterovirus-A71.

Liu Q, Long J Viruses. 2025; 17(2).

PMID: 40006936 PMC: 11861800. DOI: 10.3390/v17020181.

The Repurposing of Cellular Proteins during Enterovirus A71 Infection.

Abedeera S, Davila-Calderon J, Haddad C, Henry B, King J, Penumutchu S Viruses. 2024; 16(1).

PMID: 38257775 PMC: 10821071. DOI: 10.3390/v16010075.

Advances and Breakthroughs in IRES-Directed Translation and Replication of Picornaviruses.

Abdullah S, Wu J, Wang X, Guo H, Sun S mBio. 2023; 14(2):e0035823.

PMID: 36939331 PMC: 10127998. DOI: 10.1128/mbio.00358-23.

Picornavirus translation strategies.

Francisco-Velilla R, Embarc-Buh A, Abellan S, Martinez-Salas E FEBS Open Bio. 2022; 12(6):1125-1141.

PMID: 35313388 PMC: 9157412. DOI: 10.1002/2211-5463.13400.

RNA-Binding Proteins as Regulators of Internal Initiation of Viral mRNA Translation.

Lopez-Ulloa B, Fuentes Y, Pizarro-Ortega M, Lopez-Lastra M Viruses. 2022; 14(2).

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