The Role of Arabidopsis MYB2 in MiR399f-mediated Phosphate-starvation Response

Overview

Journal Plant Signal Behav

Specialty Biology

Date 2013 Jan 22

PMID 23333957

Citations 18

Authors

Dongwon Baek

Hyeong Cheol Park

Min Chul Kim

Dae-Jin Yun

Affiliations

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Abstract

In plants, microRNA399 (miR399) is a major regulator of phosphate (Pi) homeostasis by way of post-transcriptional mechanisms including transcript cleavage and transcriptional repression. Although miRNA genomic organization, biogenesis, and mode of action in plants are known, the regulatory mechanisms affecting miRNAs are poorly understood. We have shown that AtMYB2 functions as a transcriptional activator for miR399f expression in the context of phosphate homeostasis. AtMYB2 directly binds to a MYB-binding site in the promoter of the miR399f precursor and regulates miR399f expression. In addition, AtMYB2 transcripts are induced under Pi deficiency. The overexpression of AtMYB2 affects root system architecture (RSA), indicated by suppression of primary root growth and enhanced development of root hairs. AtMYB2 and miR399f are expressed and localized in the same tissues under Pi limitation. This study establishes that AtMYB2 regulates Pi-starvation responses (PSR) by activating of miR399f transcript, suggesting that an analysis of this miRNA promoter could reveal the existence and extent of crosstalk with other signaling mechanisms.

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References

Franco-Zorrilla J, Martin A, Leyva A, Paz-Ares J . Interaction between phosphate-starvation, sugar, and cytokinin signaling in Arabidopsis and the roles of cytokinin receptors CRE1/AHK4 and AHK3. Plant Physiol. 2005; 138(2):847-57. PMC: 1150402. DOI: 10.1104/pp.105.060517. View

Hammond J, White P . Sugar signaling in root responses to low phosphorus availability. Plant Physiol. 2011; 156(3):1033-40. PMC: 3135921. DOI: 10.1104/pp.111.175380. View

Poirier Y, Bucher M . Phosphate transport and homeostasis in Arabidopsis. Arabidopsis Book. 2012; 1:e0024. PMC: 3243343. DOI: 10.1199/tab.0024. View

Guo Y, Gan S . AtMYB2 regulates whole plant senescence by inhibiting cytokinin-mediated branching at late stages of development in Arabidopsis. Plant Physiol. 2011; 156(3):1612-9. PMC: 3135943. DOI: 10.1104/pp.111.177022. View

Raghothama K . PHOSPHATE ACQUISITION. Annu Rev Plant Physiol Plant Mol Biol. 2004; 50:665-693. DOI: 10.1146/annurev.arplant.50.1.665. View

Jiang C, Gao X, Liao L, Harberd N, Fu X . Phosphate starvation root architecture and anthocyanin accumulation responses are modulated by the gibberellin-DELLA signaling pathway in Arabidopsis. Plant Physiol. 2007; 145(4):1460-70. PMC: 2151698. DOI: 10.1104/pp.107.103788. View

Pant B, Musialak-Lange M, Nuc P, May P, Buhtz A, Kehr J . Identification of nutrient-responsive Arabidopsis and rapeseed microRNAs by comprehensive real-time polymerase chain reaction profiling and small RNA sequencing. Plant Physiol. 2009; 150(3):1541-55. PMC: 2705054. DOI: 10.1104/pp.109.139139. View

Vance C, Uhde-Stone C, Allan D . Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. New Phytol. 2021; 157(3):423-447. DOI: 10.1046/j.1469-8137.2003.00695.x. View

Lopez-Bucio J, Hernandez-Abreu E, Sanchez-Calderon L, Perez-Torres A, Rampey R, Bartel B . An auxin transport independent pathway is involved in phosphate stress-induced root architectural alterations in Arabidopsis. Identification of BIG as a mediator of auxin in pericycle cell activation. Plant Physiol. 2005; 137(2):681-91. PMC: 1065368. DOI: 10.1104/pp.104.049577. View

10.

Rubio V, Bustos R, Irigoyen M, Cardona-Lopez X, Rojas-Triana M, Paz-Ares J . Plant hormones and nutrient signaling. Plant Mol Biol. 2008; 69(4):361-73. DOI: 10.1007/s11103-008-9380-y. View

11.

Rouached H, Arpat A, Poirier Y . Regulation of phosphate starvation responses in plants: signaling players and cross-talks. Mol Plant. 2010; 3(2):288-99. DOI: 10.1093/mp/ssp120. View

12.

Fujii H, Chiou T, Lin S, Aung K, Zhu J . A miRNA involved in phosphate-starvation response in Arabidopsis. Curr Biol. 2005; 15(22):2038-43. DOI: 10.1016/j.cub.2005.10.016. View

13.

Chiou T, Aung K, Lin S, Wu C, Chiang S, Su C . Regulation of phosphate homeostasis by MicroRNA in Arabidopsis. Plant Cell. 2006; 18(2):412-21. PMC: 1356548. DOI: 10.1105/tpc.105.038943. View

14.

Yuan H, Liu D . Signaling components involved in plant responses to phosphate starvation. J Integr Plant Biol. 2008; 50(7):849-59. DOI: 10.1111/j.1744-7909.2008.00709.x. View

15.

Shin H, Shin H, Chen R, Harrison M . Loss of At4 function impacts phosphate distribution between the roots and the shoots during phosphate starvation. Plant J. 2006; 45(5):712-26. DOI: 10.1111/j.1365-313X.2005.02629.x. View

16.

Peret B, Clement M, Nussaume L, Desnos T . Root developmental adaptation to phosphate starvation: better safe than sorry. Trends Plant Sci. 2011; 16(8):442-50. DOI: 10.1016/j.tplants.2011.05.006. View

17.

Hsieh L, Lin S, Shih A, Chen J, Lin W, Tseng C . Uncovering small RNA-mediated responses to phosphate deficiency in Arabidopsis by deep sequencing. Plant Physiol. 2009; 151(4):2120-32. PMC: 2785986. DOI: 10.1104/pp.109.147280. View

18.

Pant B, Buhtz A, Kehr J, Scheible W . MicroRNA399 is a long-distance signal for the regulation of plant phosphate homeostasis. Plant J. 2007; 53(5):731-8. PMC: 2268993. DOI: 10.1111/j.1365-313X.2007.03363.x. View

19.

Rubio V, LINHARES F, Solano R, Martin A, Iglesias J, Leyva A . A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae. Genes Dev. 2001; 15(16):2122-33. PMC: 312755. DOI: 10.1101/gad.204401. View