Isolation and Characterization of the Ecdysone Receptor and Its Heterodimeric Partner Ultraspiracle Through Development in Sciara Coprophila

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 2013 Jan 17

PMID 23321980

Citations 6

Authors

Michael S Foulk

John M Waggener

Janell M Johnson

Yutaka Yamamoto

Gerald M Liew

Fyodor D Urnov

Yuki Young

Genee Lee

Heidi S Smith

Susan A Gerbi

Affiliations

Soon will be listed here.

Abstract

Regulation of DNA replication is critical, and loss of control can lead to DNA amplification. Naturally occurring, developmentally regulated DNA amplification occurs in the DNA puffs of the late larval salivary gland giant polytene chromosomes in the fungus fly, Sciara coprophila. The steroid hormone ecdysone induces DNA amplification in Sciara, and the amplification origin of DNA puff II/9A contains a putative binding site for the ecdysone receptor (EcR). We report here the isolation, cloning, and characterizing of two ecdysone receptor isoforms in Sciara (ScEcR-A and ScEcR-B) and the heterodimeric partner, ultraspiracle (ScUSP). ScEcR-A is the predominant isoform in larval tissues and ScEcR-B in adult tissues, contrary to the pattern in Drosophila. Moreover, ScEcR-A is produced at amplification but is absent just prior. We discuss these results in relation to the model of ecdysone regulation of DNA amplification.

Citing Articles

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