Direct Interaction of the Polycomb Protein with Antennapedia Regulatory Sequences in Polytene Chromosomes of Drosophila Melanogaster

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1991 Jan 1

PMID 1671215

Citations 46

Authors

B Zink

Y Engstrom

W J Gehring

R Paro

Affiliations

Soon will be listed here.

Abstract

The Polycomb (Pc) gene is responsible for the elaboration and maintenance of the expression pattern of the homeotic genes during development of Drosophila. In mutant Pc- embryos, homeotic transcripts are ectopically expressed, leading to abdominal transformations in all segments. From this it was suggested that PC+ acts as a repressor of homeotic gene transcription. We have mapped the cis-acting control sequences of the homeotic Antennapedia (Antp) gene regulated by Pc. Using Antp P1 and P2 promoter fragments linked to the E. coli lacZ reporter gene we show different expression patterns of beta-galactosidase (beta-gal) in transformed Pc+ and Pc- embryos. In addition we are able to visualize by immunocytochemical techniques on polytene chromosomes the direct binding of the Pc protein to the transposed cis-regulatory promoter fragments. However, short Antp P1 promoter constructs which are--due to position effects--ectopically activated in salivary glands, do not reveal a Pc binding signal.

Citing Articles

Polycomb safeguards imaginal disc specification through control of the Vestigial-Scalloped complex.

Brown H, Weasner B, Weasner B, Kumar J bioRxiv. 2023; .

PMID: 37090526 PMC: 10120697. DOI: 10.1101/2023.04.11.536444.

The early history of the eye-antennal disc of Drosophila melanogaster.

Weasner B, Kumar J Genetics. 2022; 221(1).

PMID: 35460415 PMC: 9071535. DOI: 10.1093/genetics/iyac041.

Control of Hox transcription factor concentration and cell-to-cell variability by an auto-regulatory switch.

Papadopoulos D, Skouloudaki K, Engstrom Y, Terenius L, Rigler R, Zechner C Development. 2019; 146(12).

PMID: 30642837 PMC: 6602345. DOI: 10.1242/dev.168179.

Polycomb and Trithorax Group Genes in .

Kassis J, Kennison J, Tamkun J Genetics. 2017; 206(4):1699-1725.

PMID: 28778878 PMC: 5560782. DOI: 10.1534/genetics.115.185116.

Expression of the polyhomeotic locus in development of Drosophila melanogaster.

DeCamillis M, Brock H Rouxs Arch Dev Biol. 2017; 203(7-8):429-438.

PMID: 28305949 DOI: 10.1007/BF00188692.

References

Paro R . Imprinting a determined state into the chromatin of Drosophila. Trends Genet. 1990; 6(12):416-21. DOI: 10.1016/0168-9525(90)90303-n. View

CARROLL S, Laymon R, McCutcheon M, Riley P, Scott M . The localization and regulation of Antennapedia protein expression in Drosophila embryos. Cell. 1986; 47(1):113-22. DOI: 10.1016/0092-8674(86)90372-7. View

Bermingham Jr J, Scott M . Developmentally regulated alternative splicing of transcripts from the Drosophila homeotic gene Antennapedia can produce four different proteins. EMBO J. 1988; 7(10):3211-22. PMC: 454718. DOI: 10.1002/j.1460-2075.1988.tb03188.x. View

Grosveld F, Van Assendelft G, Greaves D, Kollias G . Position-independent, high-level expression of the human beta-globin gene in transgenic mice. Cell. 1987; 51(6):975-85. DOI: 10.1016/0092-8674(87)90584-8. View

Zink B, Paro R . In vivo binding pattern of a trans-regulator of homoeotic genes in Drosophila melanogaster. Nature. 1989; 337(6206):468-71. DOI: 10.1038/337468a0. View

Schneuwly S, Kuroiwa A, Baumgartner P, Gehring W . Structural organization and sequence of the homeotic gene Antennapedia of Drosophila melanogaster. EMBO J. 1986; 5(4):733-9. PMC: 1166852. DOI: 10.1002/j.1460-2075.1986.tb04275.x. View

Harding K, Wedeen C, McGinnis W, Levine M . Spatially regulated expression of homeotic genes in Drosophila. Science. 1985; 229(4719):1236-42. DOI: 10.1126/science.3898362. View

Abbott M, Kaufman T . The relationship between the functional complexity and the molecular organization of the Antennapedia locus of Drosophila melanogaster. Genetics. 1986; 114(3):919-42. PMC: 1203021. DOI: 10.1093/genetics/114.3.919. View

Boulet A, Scott M . Control elements of the P2 promoter of the Antennapedia gene. Genes Dev. 1988; 2(12A):1600-14. DOI: 10.1101/gad.2.12a.1600. View

10.

Stroeher V, Gaiser J, Garber R . Alternative RNA splicing that is spatially regulated: generation of transcripts from the Antennapedia gene of Drosophila melanogaster with different protein-coding regions. Mol Cell Biol. 1988; 8(10):4143-54. PMC: 365483. DOI: 10.1128/mcb.8.10.4143-4154.1988. View

11.

Martinez-Arias A . The Antennapedia gene is required and expressed in parasegments 4 and 5 of the Drosophila embryo. EMBO J. 1986; 5(1):135-141. PMC: 1166705. DOI: 10.1002/j.1460-2075.1986.tb04187.x. View

12.

Stief A, Winter D, Stratling W, Sippel A . A nuclear DNA attachment element mediates elevated and position-independent gene activity. Nature. 1989; 341(6240):343-5. DOI: 10.1038/341343a0. View

13.

Rubin G, Spradling A . Genetic transformation of Drosophila with transposable element vectors. Science. 1982; 218(4570):348-53. DOI: 10.1126/science.6289436. View

14.

Irish V, Martinez-Arias A, Akam M . Spatial regulation of the Antennapedia and Ultrabithorax homeotic genes during Drosophila early development. EMBO J. 1989; 8(5):1527-37. PMC: 400984. DOI: 10.1002/j.1460-2075.1989.tb03537.x. View

15.

Krasnow M, Saffman E, Kornfeld K, Hogness D . Transcriptional activation and repression by Ultrabithorax proteins in cultured Drosophila cells. Cell. 1989; 57(6):1031-43. DOI: 10.1016/0092-8674(89)90341-3. View

16.

Gyurkovics H, Gausz J, Kummer J, Karch F . A new homeotic mutation in the Drosophila bithorax complex removes a boundary separating two domains of regulation. EMBO J. 1990; 9(8):2579-85. PMC: 552290. DOI: 10.1002/j.1460-2075.1990.tb07439.x. View

17.

Sato T, Denell R . Homoeosis in Drosophila: anterior and posterior transformations of Polycomb lethal embryos. Dev Biol. 1985; 110(1):53-64. DOI: 10.1016/0012-1606(85)90063-6. View

18.

Wedeen C, Harding K, Levine M . Spatial regulation of Antennapedia and bithorax gene expression by the Polycomb locus in Drosophila. Cell. 1986; 44(5):739-48. DOI: 10.1016/0092-8674(86)90840-8. View

19.

Harding K, Levine M . Gap genes define the limits of antennapedia and bithorax gene expression during early development in Drosophila. EMBO J. 1988; 7(1):205-14. PMC: 454254. DOI: 10.1002/j.1460-2075.1988.tb02801.x. View

20.

Bellen H, OKane C, Wilson C, Grossniklaus U, Pearson R, Gehring W . P-element-mediated enhancer detection: a versatile method to study development in Drosophila. Genes Dev. 1989; 3(9):1288-300. DOI: 10.1101/gad.3.9.1288. View