First Molecular Cytogenetic High Resolution Characterization of the NIH 3T3 Cell Line by Murine Multicolor Banding

Overview

Journal J Histochem Cytochem

Publisher Sage Publications

Specialty Biochemistry

Date 2013 Jan 17

PMID 23321776

Citations 30

Authors

Christine Leibiger

Nadezda Kosyakova

Hasmik Mkrtchyan

Michael Glei

Vladimir Trifonov

Thomas Liehr

Affiliations

Soon will be listed here.

Abstract

Since being established in 1963, the murine fibroblast cell line NIH 3T3 has been used in thousands of studies. NIH 3T3 immortalized spontaneously and became tetraploid shortly after its establishment. Here we report the first molecular cytogenetic characterization of NIH 3T3 using fluorescence in situ hybridization based multicolor banding (mcb). Overall, a complex rearranged karyotype presenting 16 breakpoints was characterized. Also it was possible to deduce the resulting gains and losses of copy numbers in NIH 3T3. Overall, only 1.8% of the NIH 3T3 genome is disome, 26.2% tri-, 60% tetra-, 10.8% quinta-, and 1.2% hexasome. Strikingly, the cell line gained only 4 derivative chromosomes since its first cytogenetic description in 1989. An attempt to align the observed imbalances of the studied cell line with their homologous regions in humans gave the following surprising result: NIH 3T3 shows imbalances as typically seen in human solid cancers of ectodermal origin.

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References

Weimer J, Kiechle M, Arnold N . FISH-microdissection (FISH-MD) analysis of complex chromosome rearrangements. Cytogenet Cell Genet. 2000; 88(1-2):114-8. DOI: 10.1159/000015502. View

Kasid U, Weichselbaum R, Brennan T, Mark G, Dritschilo A . Sensitivities of NIH/3T3-derived clonal cell lines to ionizing radiation: significance for gene transfer studies. Cancer Res. 1989; 49(12):3396-400. View

Weise A, Mrasek K, Fickelscher I, Claussen U, Cheung S, Cai W . Molecular definition of high-resolution multicolor banding probes: first within the human DNA sequence anchored FISH banding probe set. J Histochem Cytochem. 2008; 56(5):487-93. PMC: 2324187. DOI: 10.1369/jhc.2008.950550. View

Karst C, Trifonov V, Romanenko S, Claussen U, Mrasek K, Michel S . Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes. Int J Mol Med. 2006; 17(2):209-13. View

Pittoggi C, Renzi L, Zaccagnini G, Cimini D, Degrassi F, Giordano R . A fraction of mouse sperm chromatin is organized in nucleosomal hypersensitive domains enriched in retroposon DNA. J Cell Sci. 1999; 112 ( Pt 20):3537-48. DOI: 10.1242/jcs.112.20.3537. View

Kallioniemi A, Kallioniemi O, Sudar D, Rutovitz D, Gray J, Waldman F . Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science. 1992; 258(5083):818-21. DOI: 10.1126/science.1359641. View

Denko N, Giaccia A, Stringer J, Stambrook P . The human Ha-ras oncogene induces genomic instability in murine fibroblasts within one cell cycle. Proc Natl Acad Sci U S A. 1994; 91(11):5124-8. PMC: 43944. DOI: 10.1073/pnas.91.11.5124. View

Trifonov V, Karst C, Claussen U, Mrasek K, Michel S, Avner P . Microdissection-derived murine mcb probes from somatic cell hybrids. J Histochem Cytochem. 2005; 53(6):791-2. DOI: 10.1369/jhc.4B6598.2005. View

Liehr T, Starke H, Senger G, Melotte C, Weise A, Vermeesch J . Overrepresentation of small supernumerary marker chromosomes (sSMC) from chromosome 6 origin in cases with multiple sSMC. Am J Med Genet A. 2005; 140(1):46-51. DOI: 10.1002/ajmg.a.31048. View

10.

Gilbert N, Allan J . Distinctive higher-order chromatin structure at mammalian centromeres. Proc Natl Acad Sci U S A. 2001; 98(21):11949-54. PMC: 59820. DOI: 10.1073/pnas.211322798. View

11.

Trifonov V, Kosyakova N, Romanenko S, Stanyon R, Graphodatsky A, Liehr T . New insights into the karyotypic evolution in muroid rodents revealed by multicolor banding applying murine probes. Chromosome Res. 2010; 18(2):265-75. DOI: 10.1007/s10577-010-9110-6. View

12.

Aktas D, Utine E, Mrasek K, Weise A, von Eggeling F, Yalaz K . Derivative chromosome 1 and GLUT1 deficiency syndrome in a sibling pair. Mol Cytogenet. 2010; 3(1):10. PMC: 2887874. DOI: 10.1186/1755-8166-3-10. View

13.

Liehr T, Heller A, Starke H, Rubtsov N, Trifonov V, Mrasek K . Microdissection based high resolution multicolor banding for all 24 human chromosomes. Int J Mol Med. 2002; 9(4):335-9. View

14.

Hoque M, Soria J, Woo J, Lee T, Lee J, Jang S . Aquaporin 1 is overexpressed in lung cancer and stimulates NIH-3T3 cell proliferation and anchorage-independent growth. Am J Pathol. 2006; 168(4):1345-53. PMC: 1606549. DOI: 10.2353/ajpath.2006.050596. View

15.

Todaro G, Green H . Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 1963; 17:299-313. PMC: 2106200. DOI: 10.1083/jcb.17.2.299. View

16.

Guenatri M, Bailly D, Maison C, Almouzni G . Mouse centric and pericentric satellite repeats form distinct functional heterochromatin. J Cell Biol. 2004; 166(4):493-505. PMC: 2172221. DOI: 10.1083/jcb.200403109. View

17.

Graphodatsky A, Trifonov V, Stanyon R . The genome diversity and karyotype evolution of mammals. Mol Cytogenet. 2011; 4:22. PMC: 3204295. DOI: 10.1186/1755-8166-4-22. View

18.

Gebhart E, Liehr T . Patterns of genomic imbalances in human solid tumors (Review). Int J Oncol. 2000; 16(2):383-99. DOI: 10.3892/ijo.16.2.383. View

19.

Rieske P, Krynska B, Azizi S . Human fibroblast-derived cell lines have characteristics of embryonic stem cells and cells of neuro-ectodermal origin. Differentiation. 2005; 73(9-10):474-83. DOI: 10.1111/j.1432-0436.2005.00050.x. View