Permanent Neonatal Diabetes in INS(C94Y) Transgenic Pigs

Overview

Journal Diabetes

Specialty Endocrinology

Date 2013 Jan 1

PMID 23274907

Citations 58

Authors

Simone Renner

Christina Braun-Reichhart

Andreas Blutke

Nadja Herbach

Daniela Emrich

Elisabeth Streckel

Annegret Wunsch

Barbara Kessler

Mayuko Kurome

Andrea Bahr

Nikolai Klymiuk

Stefan Krebs

Oliver Puk

Hiroshi Nagashima

Jochen Graw

Helmut Blum

Ruediger Wanke

Eckhard Wolf

Affiliations

Soon will be listed here.

Abstract

Mutations in the insulin (INS) gene may cause permanent neonatal diabetes mellitus (PNDM). Ins2 mutant mouse models provided important insights into the disease mechanisms of PNDM but have limitations for translational research. To establish a large animal model of PNDM, we generated INS(C94Y) transgenic pigs. A line expressing high levels of INS(C94Y) mRNA (70-86% of wild-type INS transcripts) exhibited elevated blood glucose soon after birth but unaltered β-cell mass at the age of 8 days. At 4.5 months, INS(C94Y) transgenic pigs exhibited 41% reduced body weight, 72% decreased β-cell mass (-53% relative to body weight), and 60% lower fasting insulin levels compared with littermate controls. β-cells of INS(C94Y) transgenic pigs showed a marked reduction of insulin secretory granules and severe dilation of the endoplasmic reticulum. Cataract development was already visible in 8-day-old INS(C94Y) transgenic pigs and became more severe with increasing age. Diabetes-associated pathological alterations of kidney and nervous tissue were not detected during the observation period of 1 year. The stable diabetic phenotype and its rescue by insulin treatment make the INS(C94Y) transgenic pig an attractive model for insulin supplementation and islet transplantation trials, and for studying developmental consequences of maternal diabetes mellitus.

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