Existence of Glioma Stroma Mesenchymal Stemlike Cells in Korean Glioma Specimens

Overview

Journal Childs Nerv Syst

Specialty Pediatrics

Date 2013 Jan 1

PMID 23274635

Citations 19

Authors

Young Goo Kim

Soyoun Jeon

Ga-Yeong Sin

Jin-Kyoung Shim

Bo-Kyung Kim

Hye-Jin Shin

Ji-Hyun Lee

Yong-Min Huh

Su-Jae Lee

Eui-Hyun Kim

Eun Kyung Park

Se-Hoon Kim

Jong Hee Chang

Dong Seok Kim

Sun Ho Kim

Yong-Kil Hong

Seok-Gu Kang

Frederick F Lang

Affiliations

Soon will be listed here.

Abstract

Purpose: It was presented that mesenchymal stem cells (MSCs) can be isolated from western glioma specimens. However, whether MSCs exist in glioma specimens of different ethnicities is unknown. To verify the existence of MSCs in an independent cohort, we undertook studies to isolate MSCs from a group of Korean patients. We hypothesized that cells resembling MSCs that were deemed mesenchymal stemlike cells (MSLCs) exist in an independent cohort of Korean gliomas.

Methods: We cultured fresh glioma specimens using the protocols used for culturing MSCs. The cultured cells were analyzed with fluorescence-activated cell sorting (FACS) for surface markers associated with MSCs. Cultured cells were exposed to mesenchymal differentiation conditions. To presume possible locations of MSLCs in the glioma, sections of glioma were analyzed by immunofluorescent labeling for CD105, CD31, and NG2.

Results: From nine of 31 glioma specimens, we isolated cells resembling MSCs, which were deemed Korean glioma stroma MSLCs (KGS-MSLCs). KGS-MSLCs were spindle shaped and adherent to plastic. KGS-MSLCs had similar surface markers to MSCs (CD105(+), CD90(+), CD73(+), and CD45(-)). KGS-MSLCs were capable of mesenchymal differentiation and might be located around endothelial cells, pericytes, and in a disorganized perivascular area inside glioma stroma.

Conclusions: We found that cells resembling MSCs indeed exist in an independent cohort of glioma patients, as presented in western populations. We could presume that the possible location of KGS-MSLCs was in perivascular area or in glioma stroma that was a disorganized vascular niche. It might be possible that KGS-MSLCs could be one of constituent of stroma of glioma microenvironment.

Citing Articles

Role of cancer-associated mesenchymal stem cells in the tumor microenvironment: A review.

Wang K, Ding D Tzu Chi Med J. 2023; 35(1):24-30.

PMID: 36866340 PMC: 9972927. DOI: 10.4103/tcmj.tcmj_138_22.

Mesenchymal Stem-Like Cells Derived from the Ventricle More Effectively Enhance Invasiveness of Glioblastoma Than Those Derived from the Tumor.

Park J, Lee D, Shim J, Yoon S, Moon J, Kim E Yonsei Med J. 2023; 64(3):157-166.

PMID: 36825341 PMC: 9971438. DOI: 10.3349/ymj.2022.0430.

C5α secreted by tumor mesenchymal stem-like cells mediates resistance to 5-aminolevulinic acid-based photodynamic therapy against glioblastoma tumorspheres.

Park J, Oh S, Shim J, Ji Y, Moon J, Kim E J Cancer Res Clin Oncol. 2022; 149(8):4391-4402.

PMID: 36107247 DOI: 10.1007/s00432-022-04347-w.

Glioblastoma-educated mesenchymal stem-like cells promote glioblastoma infiltration via extracellular matrix remodelling in the tumour microenvironment.

Kim S, Lim E, Yoo K, Zhao Y, Kang J, Lim E Clin Transl Med. 2022; 12(8):e997.

PMID: 35908277 PMC: 9339241. DOI: 10.1002/ctm2.997.

Soluble ICAM-1 a Pivotal Communicator between Tumors and Macrophages, Promotes Mesenchymal Shift of Glioblastoma.

Yoo K, Kang J, Choi M, Suh Y, Zhao Y, Kim M Adv Sci (Weinh). 2021; 9(2):e2102768.

PMID: 34813169 PMC: 8805565. DOI: 10.1002/advs.202102768.

References

Stewart P, Farrell C, Del Maestro R . The effect of cellular microenvironment on vessels in the brain. Part 1: Vessel structure in tumour, peritumour and brain from humans with malignant glioma. Int J Radiat Biol. 1991; 60(1-2):125-30. DOI: 10.1080/09553009114551701. View

Ng H, Tse C, Lo S . Meningiomas and arachnoid cells: an immunohistochemical study of epithelial markers. Pathology. 1987; 19(3):253-7. DOI: 10.3109/00313028709066559. View

Tso C, Shintaku P, Chen J, Liu Q, Liu J, Chen Z . Primary glioblastomas express mesenchymal stem-like properties. Mol Cancer Res. 2006; 4(9):607-19. DOI: 10.1158/1541-7786.MCR-06-0005. View

Karnoub A, Dash A, Vo A, Sullivan A, Brooks M, Bell G . Mesenchymal stem cells within tumour stroma promote breast cancer metastasis. Nature. 2007; 449(7162):557-63. DOI: 10.1038/nature06188. View

Zhu L, Xiang P, Guo K, Wang A, Lu J, Tay S . Microglia/monocytes with NG2 expression have no phagocytic function in the cortex after LPS focal injection into the rat brain. Glia. 2012; 60(9):1417-26. DOI: 10.1002/glia.22362. View

Bodey B, Bodey Jr B, Siegel S, Kaiser H . Upregulation of endoglin (CD105) expression during childhood brain tumor-related angiogenesis. Anti-angiogenic therapy. Anticancer Res. 1998; 18(3A):1485-500. View

Liotta L, Kohn E . The microenvironment of the tumour-host interface. Nature. 2001; 411(6835):375-9. DOI: 10.1038/35077241. View

Robertson J, Gunter B, Somes G . Racial differences in the incidence of gliomas: a retrospective study from Memphis, Tennessee. Br J Neurosurg. 2003; 16(6):562-6. View

Nakamura K, Ito Y, Kawano Y, Kurozumi K, Kobune M, Tsuda H . Antitumor effect of genetically engineered mesenchymal stem cells in a rat glioma model. Gene Ther. 2004; 11(14):1155-64. DOI: 10.1038/sj.gt.3302276. View

10.

Lee J, Kotliarova S, Kotliarov Y, Li A, Su Q, Donin N . Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines. Cancer Cell. 2006; 9(5):391-403. DOI: 10.1016/j.ccr.2006.03.030. View

11.

Yao Y, Kubota T, Takeuchi H, Sato K . Prognostic significance of microvessel density determined by an anti-CD105/endoglin monoclonal antibody in astrocytic tumors: comparison with an anti-CD31 monoclonal antibody. Neuropathology. 2005; 25(3):201-6. DOI: 10.1111/j.1440-1789.2005.00632.x. View

12.

Freije W, Castro-Vargas F, Fang Z, Horvath S, Cloughesy T, Liau L . Gene expression profiling of gliomas strongly predicts survival. Cancer Res. 2004; 64(18):6503-10. DOI: 10.1158/0008-5472.CAN-04-0452. View

13.

Verhaak R, Hoadley K, Purdom E, Wang V, Qi Y, Wilkerson M . Integrated genomic analysis identifies clinically relevant subtypes of glioblastoma characterized by abnormalities in PDGFRA, IDH1, EGFR, and NF1. Cancer Cell. 2010; 17(1):98-110. PMC: 2818769. DOI: 10.1016/j.ccr.2009.12.020. View

14.

Singh S, Clarke I, Terasaki M, Bonn V, Hawkins C, Squire J . Identification of a cancer stem cell in human brain tumors. Cancer Res. 2003; 63(18):5821-8. View

15.

Dirks P . Brain tumor stem cells. Biol Blood Marrow Transplant. 2005; 11(2 Suppl 2):12-3. DOI: 10.1016/j.bbmt.2004.11.004. View

16.

Crisan M, Chen C, Corselli M, Andriolo G, Lazzari L, Peault B . Perivascular multipotent progenitor cells in human organs. Ann N Y Acad Sci. 2009; 1176:118-23. DOI: 10.1111/j.1749-6632.2009.04967.x. View

17.

Kang S, Shinojima N, Hossain A, Gumin J, Yong R, Colman H . Isolation and perivascular localization of mesenchymal stem cells from mouse brain. Neurosurgery. 2010; 67(3):711-20. PMC: 3644957. DOI: 10.1227/01.NEU.0000377859.06219.78. View

18.

Kim S, Kang S, Park N, Mok H, Huh Y, Lee S . Presence of glioma stroma mesenchymal stem cells in a murine orthotopic glioma model. Childs Nerv Syst. 2011; 27(6):911-22. DOI: 10.1007/s00381-011-1396-y. View

19.

Kong B, Park N, Shim J, Kim B, Shin H, Lee J . Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens. Childs Nerv Syst. 2012; 29(2):217-29. DOI: 10.1007/s00381-012-1964-9. View

20.

Zannettino A, Paton S, Arthur A, Khor F, Itescu S, Gimble J . Multipotential human adipose-derived stromal stem cells exhibit a perivascular phenotype in vitro and in vivo. J Cell Physiol. 2007; 214(2):413-21. DOI: 10.1002/jcp.21210. View