Subversion of Autophagy in Adherent Invasive Escherichia Coli-infected Neutrophils Induces Inflammation and Cell Death

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Dec 29

PMID 23272151

Citations 35

Authors

Abderrahman Chargui

Annabelle Cesaro

Sanda Mimouna

Mohamed Fareh

Patrick Brest

Philippe Naquet

Arlette Darfeuille-Michaud

Xavier Hebuterne

Baharia Mograbi

Valerie Vouret-Craviari

Paul Hofman

Affiliations

Soon will be listed here.

Abstract

Invading bacteria are recognized, captured and killed by a specialized form of autophagy, called xenophagy. Recently, defects in xenophagy in Crohn's disease (CD) have been implicated in the pathogenesis of human chronic inflammatory diseases of uncertain etiology of the gastrointestinal tract. We show here that pathogenic adherent-invasive Escherichia coli (AIEC) isolated from CD patients are able to adhere and invade neutrophils, which represent the first line of defense against bacteria. Of particular interest, AIEC infection of neutrophil-like PLB-985 cells blocked autophagy at the autolysosomal step, which allowed intracellular survival of bacteria and exacerbated interleukin-8 (IL-8) production. Interestingly, this block in autophagy correlated with the induction of autophagic cell death. Likewise, stimulation of autophagy by nutrient starvation or rapamycin treatment reduced intracellular AIEC survival and IL-8 production. Finally, treatment with an inhibitor of autophagy decreased cell death of AIEC-infected neutrophil-like PLB-985 cells. In conclusion, excessive autophagy in AIEC infection triggered cell death of neutrophils.

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