Occurrence and Molecular Characterization of Hysterothylacium Aduncum (Nematoda: Anisakidae) from Merlangius Merlangus Euxinus and Trachurus Trachurus off the Turkish Coast of Black Sea

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2012 Dec 15

PMID 23239095

Citations 5

Authors

Gokmen Zafer Pekmezci

Cenk Soner Bolukbas

Ali Tumay Gurler

Ertan Emek Onuk

Affiliations

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Abstract

A total of 286 larval forms of Hysterothylacium aduncum were collected from Merlangius merlangus euxinus and Trachurus trachurus captured at different sites of the Black Sea coast of Turkey. Prevalence of H. aduncum in M. merlangus euxinus and T. trachurus was 37.4 and 29.3 %, respectively. The fourth-stage larvae from M. merlangus euxinus and T. trachurus of H. aduncum were characterized genetically using a molecular approach. The ribosomal DNA (rDNA) internal transcribed spacer (ITS) region (ITS-1, 5.8S subunit, ITS-2) was amplified and sequenced. Two isolates of H. aduncum obtained from M. merlangus euxinus and T. trachurus in Black Sea showed a 100 % nucleotide similarity. Pairwise comparison between the entire ITS fragment including ITS-1, 5.8S, ITS-2 sequences of the H. adumcum isolates of M. merlangus euxinus and T. trachurus from Black Sea (Turkey, JX413596-JX413597) and other H. adumcum isolates from Baltic Sea (Poland, AJ937672), North Sea (Denmark, HM598666), Mediterranean Sea (Tunisia, HQ270427), Japan Sea (Japan, AB277826), Adriatic Sea (Croatia, JQ934878), East Greenland Sea, English Channel, Bay of Biscay, Adriatic Sea, and North Sea showed differences ranging from 0.1 to 0.7 %. With the present study, larvae of H. aduncum infecting M. merlangus euxinus and T. trachurus caught off the Black Sea, Turkey were characterized for the first time by sequencing of the ITS rDNA.

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References

DAmelio S, Mathiopoulos K, Santos C, Pugachev O, Webb S, Picanco M . Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism. Int J Parasitol. 2000; 30(2):223-6. DOI: 10.1016/s0020-7519(99)00178-2. View

Shih H . Parasitic helminth fauna of the cutlass fish, Trichiurus lepturus L., and the differentiation of four anisakid nematode third-stage larvae by nuclear ribosomal DNA sequences. Parasitol Res. 2004; 93(3):188-95. DOI: 10.1007/s00436-004-1095-7. View

Vardic Smrzlic I, Valic D, Kapetanovic D, Kurtovic B, Teskeredzic E . Molecular characterisation of Anisakidae larvae from fish in Adriatic Sea. Parasitol Res. 2012; 111(6):2385-91. DOI: 10.1007/s00436-012-3094-4. View

Umehara A, Kawakami Y, Araki J, Uchida A . Multiplex PCR for the identification of Anisakis simplex sensu stricto, Anisakis pegreffii and the other anisakid nematodes. Parasitol Int. 2007; 57(1):49-53. DOI: 10.1016/j.parint.2007.08.003. View

Mattiucci S, Nascetti G . Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host-parasite co-evolutionary processes. Adv Parasitol. 2008; 66:47-148. DOI: 10.1016/S0065-308X(08)00202-9. View

Klimpel S, Kleinertz S, Hanel R, Ruckert S . Genetic variability in Hysterothylacium aduncum, a raphidascarid nematode isolated from sprat (Sprattus sprattus) of different geographical areas of the northeastern Atlantic. Parasitol Res. 2007; 101(5):1425-30. DOI: 10.1007/s00436-007-0662-0. View

Klimpel S, Ruckert S . Life cycle strategy of Hysterothylacium aduncum to become the most abundant anisakid fish nematode in the North Sea. Parasitol Res. 2005; 97(2):141-9. DOI: 10.1007/s00436-005-1407-6. View

Zhu X, Podolska M, Liu J, Yu H, Chen H, Lin Z . Identification of anisakid nematodes with zoonotic potential from Europe and China by single-strand conformation polymorphism analysis of nuclear ribosomal DNA. Parasitol Res. 2007; 101(6):1703-7. DOI: 10.1007/s00436-007-0699-0. View

Zhu X, Gasser R, Podolska M, Chilton N . Characterisation of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. Int J Parasitol. 1999; 28(12):1911-21. DOI: 10.1016/s0020-7519(98)00150-7. View

10.

Valero A, Terrados S, Diaz V, Reguera V, Lozano J . Determination of IgE in the serum of patients with allergic reactions to four species of fish-parasite anisakids. J Investig Allergol Clin Immunol. 2003; 13(2):94-8. View

11.

Rozsa L, Reiczigel J, Majoros G . Quantifying parasites in samples of hosts. J Parasitol. 2000; 86(2):228-32. DOI: 10.1645/0022-3395(2000)086[0228:QPISOH]2.0.CO;2. View

12.

Felsenstein J . CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP. Evolution. 2017; 39(4):783-791. DOI: 10.1111/j.1558-5646.1985.tb00420.x. View

13.

Mattiucci S, Abaunza P, Damiano S, Garcia A, Santos M, Nascetti G . Distribution of Anisakis larvae, identified by genetic markers, and their use for stock characterization of demersal and pelagic fish from European waters: an update. J Helminthol. 2007; 81(2):117-27. DOI: 10.1017/S0022149X07754718. View

14.

Navone G, Sardella N, Timi J . Larvae and adults of Hysterothylacium aduncum (Rudolphi, 1802) (Nematoda: Anisakidae) in fishes and crustaceans in the south west Atlantic. Parasite. 1998; 5(2):127-36. DOI: 10.1051/parasite/1998052127. View

15.

Farjallah S, Ben Slimane B, Busi M, Paggi L, Amor N, Blel H . Occurrence and molecular identification of Anisakis spp. from the North African coasts of Mediterranean Sea. Parasitol Res. 2007; 102(3):371-9. DOI: 10.1007/s00436-007-0771-9. View

16.

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

17.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S . MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011; 28(10):2731-9. PMC: 3203626. DOI: 10.1093/molbev/msr121. View

18.

Pontes T, DAmelio S, Costa G, Paggi L . Molecular characterization of larval anisakid nematodes from marine fishes of Madeira by a PCR-based approach, with evidence for a new species. J Parasitol. 2006; 91(6):1430-4. DOI: 10.1645/GE-565R1.1. View

19.

Thompson J, Higgins D, Gibson T . CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22(22):4673-80. PMC: 308517. DOI: 10.1093/nar/22.22.4673. View

20.

Amor N, Farjallah S, Merella P, Said K, Ben Slimane B . Molecular characterization of Hysterothylacium aduncum (Nematoda: Raphidascaridae) from different fish caught off the Tunisian coast based on nuclear ribosomal DNA sequences. Parasitol Res. 2011; 109(5):1429-37. DOI: 10.1007/s00436-011-2391-7. View