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Optogenetic Control of Transcription in Zebrafish

Overview
Journal PLoS One
Specialties General Medicine
Science
Date 2012 Dec 11
PMID 23226369
Citations 46
Authors
Hongtao Liu
Gustavo Gomez
Sophia Lin
Shuo Lin
Chentao Lin
Affiliations
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Abstract

Light inducible protein-protein interactions are powerful tools to manipulate biological processes. Genetically encoded light-gated proteins for controlling precise cellular behavior are a new and promising technology, called optogenetics. Here we exploited the blue light-induced transcription system in yeast and zebrafish, based on the blue light dependent interaction between two plant proteins, blue light photoreceptor Cryptochrome 2 (CRY2) and the bHLH transcription factor CIB1 (CRY-interacting bHLH 1). We demonstrate the utility of this system by inducing rapid transcription suppression and activation in zebrafish.

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References
1.
Wu L, Yang H . CRYPTOCHROME 1 is implicated in promoting R protein-mediated plant resistance to Pseudomonas syringae in Arabidopsis. Mol Plant. 2010; 3(3):539-48. DOI: 10.1093/mp/ssp107. View
2.
Jeong R, Chandra-Shekara A, Roy Barman S, Navarre D, Klessig D, Kachroo A . Cryptochrome 2 and phototropin 2 regulate resistance protein-mediated viral defense by negatively regulating an E3 ubiquitin ligase. Proc Natl Acad Sci U S A. 2010; 107(30):13538-43. PMC: 2922132. DOI: 10.1073/pnas.1004529107. View
3.
Levskaya A, Weiner O, Lim W, Voigt C . Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009; 461(7266):997-1001. PMC: 2989900. DOI: 10.1038/nature08446. View
4.
Toettcher J, Gong D, Lim W, Weiner O . Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011; 8(10):837-9. PMC: 3184382. DOI: 10.1038/nmeth.1700. View
5.
Liu H, Yu X, Li K, Klejnot J, Yang H, Lisiero D . Photoexcited CRY2 interacts with CIB1 to regulate transcription and floral initiation in Arabidopsis. Science. 2008; 322(5907):1535-9. DOI: 10.1126/science.1163927. View