Pre-conditioned Mesenchymal Stem Cells Ameliorate Renal Ischemic Injury in Rats by Augmented Survival and Engraftment

Overview

Journal J Transl Med

Publisher Biomed Central

Specialties Biomedical Engineering
General Medicine

Date 2012 Dec 11

PMID 23217165

Citations 23

Authors

Muhammad Shareef Masoud

Sanam Saiqa Anwar

Muhammad Zeeshan Afzal

Azra Mehmood

Shaheen N Khan

Sheikh Riazuddin

Affiliations

Soon will be listed here.

Abstract

Background: Ischemia is the major cause of acute kidney injury (AKI), associated with high mortality and morbidity. Mesenchymal stem cells (MSCs) have multilineage differentiation potential and can be a potent therapeutic option for the cure of AKI.

Methods: MSCs were cultured in four groups SNAP (S-nitroso N-acetyl penicillamine), SNAP + Methylene Blue (MB), MB and a control for in vitro analysis. Cultured MSCs were pre-conditioned with either SNAP (100 μM) or MB (1 μM) or both for 6 hours. Renal ischemia was induced in four groups (as in in vitro study) of rats by clamping the left renal padicle for 45 minutes and then different pre-conditioned stem cells were transplanted.

Results: We report that pre-conditioning of MSCs with SNAP enhances their proliferation, survival and engraftment in ischemic kidney. Rat MSCs pre-conditioned with SNAP decreased cell apoptosis and increased proliferation and cytoprotective genes' expression in vitro. Our in vivo data showed enhanced survival and engraftment, proliferation, reduction in fibrosis, significant improvement in renal function and higher expression of pro-survival and pro-angiogenic factors in ischemic renal tissue in SNAP pre-conditioned group of animals. Cytoprotective effects of SNAP pre-conditioning were abrogated by MB, an inhibitor of nitric oxide synthase (NOS) and guanylate cyclase.

Conclusion: The results of these studies demonstrate that SNAP pre-conditioning might be useful to enhance therapeutic potential of MSCs in attenuating renal ischemia reperfusion injury.

Citing Articles

Advances in the Treatment of Kidney Disorders using Mesenchymal Stem Cells.

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Optimization strategies of mesenchymal stem cell-based therapy for acute kidney injury.

Fu Z, Zhang Y, Geng X, Chi K, Liu C, Song C Stem Cell Res Ther. 2023; 14(1):116.

PMID: 37122024 PMC: 10150535. DOI: 10.1186/s13287-023-03351-2.

Mesenchymal Stem Cell Therapy in Kidney Diseases: Potential and Challenges.

Chen F, Chen N, Xia C, Wang H, Shao L, Zhou C Cell Transplant. 2023; 32:9636897231164251.

PMID: 37013255 PMC: 10074620. DOI: 10.1177/09636897231164251.

A review on renal autologous cell transplantation: an investigational approach towards chronic kidney disease.

Copur S, Yavuz F, Covic A, Kanbay M Int Urol Nephrol. 2023; 55(10):2539-2544.

PMID: 36971874 DOI: 10.1007/s11255-023-03574-5.

Enhancing the Therapeutic Potential of Mesenchymal Stromal Cell-Based Therapies with an Anti-Fibrotic Agent for the Treatment of Chronic Kidney Disease.

Li Y, Ricardo S, Samuel C Int J Mol Sci. 2022; 23(11).

PMID: 35682717 PMC: 9181689. DOI: 10.3390/ijms23116035.

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