Nuclear Aggregation of Olfactory Receptor Genes Governs Their Monogenic Expression

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 2012 Nov 13

PMID 23141535

Citations 192

Authors

E Josephine Clowney

Mark A LeGros

Colleen P Mosley

Fiona G Clowney

Eirene C Markenskoff-Papadimitriou

Markko Myllys

Gilad Barnea

Carolyn A Larabell

Stavros Lomvardas

Affiliations

Soon will be listed here.

Abstract

Gene positioning and regulation of nuclear architecture are thought to influence gene expression. Here, we show that, in mouse olfactory neurons, silent olfactory receptor (OR) genes from different chromosomes converge in a small number of heterochromatic foci. These foci are OR exclusive and form in a cell-type-specific and differentiation-dependent manner. The aggregation of OR genes is developmentally synchronous with the downregulation of lamin b receptor (LBR) and can be reversed by ectopic expression of LBR in mature olfactory neurons. LBR-induced reorganization of nuclear architecture and disruption of OR aggregates perturbs the singularity of OR transcription and disrupts the targeting specificity of the olfactory neurons. Our observations propose spatial sequestering of heterochromatinized OR family members as a basis of monogenic and monoallelic gene expression.

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