Genetic Diversity of Burkholderia Contaminans Isolates from Cystic Fibrosis Patients in Argentina

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2012 Nov 9

PMID 23135937

Citations 19

Authors

Pablo Martina

Marisa Bettiol

Cecilia Vescina

Patricia Montanaro

M Constanza Mannino

Claudia I Prieto

Carlos Vay

Dieter Naumann

Juergen Schmitt

Osvaldo Yantorno

Antonio Lagares

Alejandra Bosch

Affiliations

Soon will be listed here.

Abstract

A total of 120 Burkholderia cepacia complex isolates collected during 2004-2010 from 66 patients in two cystic fibrosis reference centers in Argentina were analyzed. Burkholderia contaminans was the species most frequently recovered (57.6%), followed by Burkholderia cenocepacia (15%), a species distribution not reported so far. The recA-PCR-based techniques applied to the B. contaminans isolates revealed that 85% of the population carried the recA-ST-71 allele. Our results showed the utility of BOX-PCR genotyping in analyzing B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.

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References

Mahenthiralingam E, Vandamme P, CAMPBELL M, Henry D, Gravelle A, Wong L . Infection with Burkholderia cepacia complex genomovars in patients with cystic fibrosis: virulent transmissible strains of genomovar III can replace Burkholderia multivorans. Clin Infect Dis. 2001; 33(9):1469-75. DOI: 10.1086/322684. View

Bosch A, Minan A, Vescina C, Degrossi J, Gatti B, Montanaro P . Fourier transform infrared spectroscopy for rapid identification of nonfermenting gram-negative bacteria isolated from sputum samples from cystic fibrosis patients. J Clin Microbiol. 2008; 46(8):2535-46. PMC: 2519462. DOI: 10.1128/JCM.02267-07. View

Jorda-Vargas L, Degrossi J, Castaneda N, DAquino M, Valvano M, Procopio A . Prevalence of indeterminate genetic species of Burkholderia cepacia complex in a cystic fibrosis center in Argentina. J Clin Microbiol. 2008; 46(3):1151-2. PMC: 2268362. DOI: 10.1128/JCM.01595-07. View

Detsika M, Corkill J, Magalhaes M, Glendinning K, Hart C, Winstanley C . Molecular typing of, and distribution of genetic markers among, Burkholderia cepacia complex isolates from Brazil. J Clin Microbiol. 2003; 41(9):4148-53. PMC: 193793. DOI: 10.1128/JCM.41.9.4148-4153.2003. View

Currie B, Gal D, Mayo M, Ward L, Godoy D, Spratt B . Using BOX-PCR to exclude a clonal outbreak of melioidosis. BMC Infect Dis. 2007; 7:68. PMC: 1925088. DOI: 10.1186/1471-2334-7-68. View

Bernhardt S, Spilker T, Coffey T, LiPuma J . Burkholderia cepacia complex in cystic fibrosis: frequency of strain replacement during chronic infection. Clin Infect Dis. 2003; 37(6):780-5. DOI: 10.1086/377541. View

Versalovic J, Koeuth T, Lupski J . Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 1991; 19(24):6823-31. PMC: 329316. DOI: 10.1093/nar/19.24.6823. View

Vermis K, Coenye T, Mahenthiralingam E, Nelis H, Vandamme P . Evaluation of species-specific recA-based PCR tests for genomovar level identification within the Burkholderia cepacia complex. J Med Microbiol. 2002; 51(11):937-940. DOI: 10.1099/0022-1317-51-11-937. View

Yang J, Spilker T, LiPuma J . Simultaneous coinfection by multiple strains during Burkholderia cepacia complex infection in cystic fibrosis. Diagn Microbiol Infect Dis. 2006; 54(2):95-8. DOI: 10.1016/j.diagmicrobio.2005.08.020. View

10.

LiPuma J . The changing microbial epidemiology in cystic fibrosis. Clin Microbiol Rev. 2010; 23(2):299-323. PMC: 2863368. DOI: 10.1128/CMR.00068-09. View

11.

Coenye T, Spilker T, Martin A, LiPuma J . Comparative assessment of genotyping methods for epidemiologic study of Burkholderia cepacia genomovar III. J Clin Microbiol. 2002; 40(9):3300-7. PMC: 130787. DOI: 10.1128/JCM.40.9.3300-3307.2002. View

12.

Biddick R, Spilker T, Martin A, LiPuma J . Evidence of transmission of Burkholderia cepacia, Burkholderia multivorans and Burkholderia dolosa among persons with cystic fibrosis. FEMS Microbiol Lett. 2003; 228(1):57-62. DOI: 10.1016/S0378-1097(03)00724-9. View

13.

Yang L, Jelsbak L, Marvig R, Damkiaer S, Workman C, Rau M . Evolutionary dynamics of bacteria in a human host environment. Proc Natl Acad Sci U S A. 2011; 108(18):7481-6. PMC: 3088582. DOI: 10.1073/pnas.1018249108. View

14.

Assaad W, Magalhaes M, Plesa M, Hart C, Cornelis P, Winstanley C . Identical Burkholderia cepacia complex strain types isolated from multiple patients attending a hospital in Brazil. J Med Microbiol. 2006; 55(Pt 2):247-249. DOI: 10.1099/jmm.0.46321-0. View

15.

Vanlaere E, Baldwin A, Gevers D, Henry D, De Brandt E, LiPuma J . Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp. nov. and Burkholderia lata sp. nov. Int J Syst Evol Microbiol. 2009; 59(Pt 1):102-11. DOI: 10.1099/ijs.0.001123-0. View

16.

Smith E, Buckley D, Wu Z, Saenphimmachak C, Hoffman L, DArgenio D . Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients. Proc Natl Acad Sci U S A. 2006; 103(22):8487-92. PMC: 1482519. DOI: 10.1073/pnas.0602138103. View

17.

Baldwin A, Mahenthiralingam E, Thickett K, Honeybourne D, Maiden M, Govan J . Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex. J Clin Microbiol. 2005; 43(9):4665-73. PMC: 1234123. DOI: 10.1128/JCM.43.9.4665-4673.2005. View

18.

Mahenthiralingam E, Bischof J, Byrne S, Radomski C, Davies J, Av-Gay Y . DNA-Based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. J Clin Microbiol. 2000; 38(9):3165-73. PMC: 87345. DOI: 10.1128/JCM.38.9.3165-3173.2000. View

19.

Zhou J, Garber E, Desai M, Saiman L . Compliance of clinical microbiology laboratories in the United States with current recommendations for processing respiratory tract specimens from patients with cystic fibrosis. J Clin Microbiol. 2006; 44(4):1547-9. PMC: 1448617. DOI: 10.1128/JCM.44.4.1547-1549.2006. View

20.

Jelsbak L, Krogh Johansen H, Frost A, Thogersen R, Thomsen L, Ciofu O . Molecular epidemiology and dynamics of Pseudomonas aeruginosa populations in lungs of cystic fibrosis patients. Infect Immun. 2007; 75(5):2214-24. PMC: 1865789. DOI: 10.1128/IAI.01282-06. View