Using BOX-PCR to Exclude a Clonal Outbreak of Melioidosis

Overview

Journal BMC Infect Dis

Publisher Biomed Central

Specialty Infectious Diseases

Date 2007 Jul 3

PMID 17603903

Citations 33

Authors

Bart J Currie

Daniel Gal

Mark Mayo

Linda Ward

Daniel Godoy

Brian G Spratt

John J LiPuma

Affiliations

Soon will be listed here.

Abstract

Background: Although melioidosis in endemic regions is usually caused by a diverse range of Burkholderia pseudomallei strains, clonal outbreaks from contaminated potable water have been described. Furthermore B. pseudomallei is classified as a CDC Group B bioterrorism agent. Ribotyping, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) have been used to identify genetically related B. pseudomallei isolates, but they are time consuming and technically challenging for many laboratories.

Methods: We have adapted repetitive sequence typing using a BOX A1R primer for typing B. pseudomallei and compared BOX-PCR fingerprinting results on a wide range of well-characterized B. pseudomallei isolates with MLST and PFGE performed on the same isolates.

Results: BOX-PCR typing compared favourably with MLST and PFGE performed on the same isolates, both discriminating between the majority of multilocus sequence types and showing relatedness between epidemiologically linked isolates from various outbreak clusters.

Conclusion: Our results suggest that BOX-PCR can be used to exclude a clonal outbreak of melioidosis within 10 hours of receiving the bacterial strains.

Citing Articles

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References

Inglis T, Garrow S, Henderson M, Clair A, Sampson J, OReilly L . Burkholderia pseudomallei traced to water treatment plant in Australia. Emerg Infect Dis. 2000; 6(1):56-9. PMC: 2627980. DOI: 10.3201/eid0601.000110. View

. Imported melioidosis--South Florida, 2005. MMWR Morb Mortal Wkly Rep. 2006; 55(32):873-6. View

Hsueh P, Teng L, Lee L, Yu C, Yang P, Ho S . Melioidosis: an emerging infection in Taiwan?. Emerg Infect Dis. 2001; 7(3):428-33. PMC: 2631793. DOI: 10.3201/eid0703.010310. View

Currie B, Mayo M, Anstey N, Donohoe P, Haase A, Kemp D . A cluster of melioidosis cases from an endemic region is clonal and is linked to the water supply using molecular typing of Burkholderia pseudomallei isolates. Am J Trop Med Hyg. 2001; 65(3):177-9. DOI: 10.4269/ajtmh.2001.65.177. View

Coenye T, Spilker T, Martin A, LiPuma J . Comparative assessment of genotyping methods for epidemiologic study of Burkholderia cepacia genomovar III. J Clin Microbiol. 2002; 40(9):3300-7. PMC: 130787. DOI: 10.1128/JCM.40.9.3300-3307.2002. View

Godoy D, Randle G, Simpson A, Aanensen D, Pitt T, Kinoshita R . Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei. J Clin Microbiol. 2003; 41(5):2068-79. PMC: 154742. DOI: 10.1128/JCM.41.5.2068-2079.2003. View

White N . Melioidosis. Lancet. 2003; 361(9370):1715-22. DOI: 10.1016/s0140-6736(03)13374-0. View

Kumar S, Tamura K, Nei M . MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform. 2004; 5(2):150-63. DOI: 10.1093/bib/5.2.150. View

Gal D, Mayo M, Smith-Vaughan H, Dasari P, McKinnon M, Jacups S . Contamination of hand wash detergent linked to occupationally acquired melioidosis. Am J Trop Med Hyg. 2004; 71(3):360-2. View

10.

Golledge C, Chin W, Tribe A, Condon R, Ashdown L . A case of human melioidosis originating in south-west Western Australia. Med J Aust. 1992; 157(5):332-4. DOI: 10.5694/j.1326-5377.1992.tb137192.x. View

11.

Sexton M, Goebel L, Godfrey A, Choawagul W, White N, Woods D . Ribotype analysis of Pseudomonas pseudomallei isolates. J Clin Microbiol. 1993; 31(2):238-43. PMC: 262742. DOI: 10.1128/jcm.31.2.238-243.1993. View

12.

Currie B, Smith-Vaughan H, Golledge C, Buller N, Sriprakash K, Kemp D . Pseudomonas pseudomallei isolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping. Epidemiol Infect. 1994; 113(2):307-12. PMC: 2271530. DOI: 10.1017/s0950268800051736. View

13.

Haase A, Smith-Vaughan H, Melder A, Wood Y, Janmaat A, Gilfedder J . Subdivision of Burkholderia pseudomallei ribotypes into multiple types by random amplified polymorphic DNA analysis provides new insights into epidemiology. J Clin Microbiol. 1995; 33(7):1687-90. PMC: 228250. DOI: 10.1128/jcm.33.7.1687-1690.1995. View

14.

Cheng A, Godoy D, Mayo M, Gal D, Spratt B, Currie B . Isolates of Burkholderia pseudomallei from Northern Australia are distinct by multilocus sequence typing, but strain types do not correlate with clinical presentation. J Clin Microbiol. 2004; 42(12):5477-83. PMC: 535284. DOI: 10.1128/JCM.42.12.5477-5483.2004. View

15.

Cheng A, Currie B . Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev. 2005; 18(2):383-416. PMC: 1082802. DOI: 10.1128/CMR.18.2.383-416.2005. View

16.

Rolim D, Vilar D, Sousa A, Miralles I, de Oliveira D, Harnett G . Melioidosis, northeastern Brazil. Emerg Infect Dis. 2005; 11(9):1458-60. PMC: 3310637. DOI: 10.3201/eid1109.050493. View

17.

Cheng A, Jacups S, Gal D, Mayo M, Currie B . Extreme weather events and environmental contamination are associated with case-clusters of melioidosis in the Northern Territory of Australia. Int J Epidemiol. 2005; 35(2):323-9. DOI: 10.1093/ije/dyi271. View

18.

Liu Y, Loh J, Aw L, Yap E, Lee M, Ooi E . Rapid molecular typing of Burkholderia pseudomallei, isolated in an outbreak of melioidosis in Singapore in 2004, based on variable-number tandem repeats. Trans R Soc Trop Med Hyg. 2005; 100(7):687-92. DOI: 10.1016/j.trstmh.2005.08.017. View

19.

Vesaratchavest M, Tumapa S, Day N, Wuthiekanun V, Chierakul W, Holden M . Nonrandom distribution of Burkholderia pseudomallei clones in relation to geographical location and virulence. J Clin Microbiol. 2006; 44(7):2553-7. PMC: 1489466. DOI: 10.1128/JCM.00629-06. View

20.

Leelayuwat C, Romphruk A, Lulitanond A, Trakulsomboon S, Thamlikitkul V . Genotype analysis of Burkholderia pseudomallei using randomly amplified polymorphic DNA (RAPD): indicative of genetic differences amongst environmental and clinical isolates. Acta Trop. 2000; 77(2):229-37. DOI: 10.1016/s0001-706x(00)00137-6. View