In Vitro CO2-induced ROS Production Impairs Cell Cycle in SH-SY5Y Neuroblastoma Cells

Overview

Journal Pediatr Surg Int

Specialties General Surgery
Pediatrics

Date 2012 Nov 6

PMID 23124131

Citations 4

Authors

Angela Simona Montalto

Monica Curro

Tiziana Russo

Giuseppa Visalli

Pietro Impellizzeri

Pietro Antonuccio

Salvatore Arena

Francesca Astra Borruto

Gianfranco Scalfari

Riccardo Ientile

Carmelo Romeo

Affiliations

Soon will be listed here.

Abstract

Purpose: We evaluated in vitro the role of CO(2)-induced oxidative stress on the expression of proteins involved in cell-cycle regulation of neuroblastoma cells.

Methods: SH-SY5Y cells were exposed to CO(2) at 15 mmHg pressure (100 %) for 4 h and then moved to normal condition for 24 h. Control cells were maintained in 5 % CO(2) for the same time. ROS production was determined by fluorescent staining with H2DCF-DA. DNA damage was measured by COMET assay. p53 protein expression was analyzed by western blot and confocal laser scanning microscopy was used to evaluate its sub-cellular localization. Cyclin expression was quantified by real-time PCR and western blot. Cell-cycle analysis was performed by FACS.

Results: CO(2) incubation was associated with an increase in ROS production (p < 0.01), cell DNA damage mainly after 24 h (12 % increase of tail DNA content and 4-fold increase of tail length) and a significant up-regulation in p53 expression at 24 h with an intense nuclear staining. In CO(2)-treated cells, we observed an S-phase arrest in correlation with a reduction of cyclin B1 expression.

Conclusions: In vitro-simulated pneumoperitoneum environment with CO(2) induces oxidative stress and cell DNA damage, leading to p53 up-regulation involved in cell-cycle arrest of neuroblastoma cells.

Citing Articles

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CO pneumoperitoneum effects on proliferation and apoptosis in two different neuroblastoma cell lines.

Curro M, Arena S, Montalto A, Perrone P, Di Fabrizio D, Bertuccio M Pediatr Surg Int. 2022; 38(3):457-464.

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Hypoxia-Dependent Expression of TG2 Isoforms in Neuroblastoma Cells as Consequence of Different MYCN Amplification Status.

Curro M, Ferlazzo N, Giunta M, Montalto A, Russo T, Arena S Int J Mol Sci. 2020; 21(4).

PMID: 32085516 PMC: 7072980. DOI: 10.3390/ijms21041364.

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