» Articles » PMID: 23124131

In Vitro CO2-induced ROS Production Impairs Cell Cycle in SH-SY5Y Neuroblastoma Cells

Overview
Date 2012 Nov 6
PMID 23124131
Citations 4
Authors
Affiliations
Soon will be listed here.
Abstract

Purpose: We evaluated in vitro the role of CO(2)-induced oxidative stress on the expression of proteins involved in cell-cycle regulation of neuroblastoma cells.

Methods: SH-SY5Y cells were exposed to CO(2) at 15 mmHg pressure (100 %) for 4 h and then moved to normal condition for 24 h. Control cells were maintained in 5 % CO(2) for the same time. ROS production was determined by fluorescent staining with H2DCF-DA. DNA damage was measured by COMET assay. p53 protein expression was analyzed by western blot and confocal laser scanning microscopy was used to evaluate its sub-cellular localization. Cyclin expression was quantified by real-time PCR and western blot. Cell-cycle analysis was performed by FACS.

Results: CO(2) incubation was associated with an increase in ROS production (p < 0.01), cell DNA damage mainly after 24 h (12 % increase of tail DNA content and 4-fold increase of tail length) and a significant up-regulation in p53 expression at 24 h with an intense nuclear staining. In CO(2)-treated cells, we observed an S-phase arrest in correlation with a reduction of cyclin B1 expression.

Conclusions: In vitro-simulated pneumoperitoneum environment with CO(2) induces oxidative stress and cell DNA damage, leading to p53 up-regulation involved in cell-cycle arrest of neuroblastoma cells.

Citing Articles

The Tissue Response to Hypoxia: How Therapeutic Carbon Dioxide Moves the Response toward Homeostasis and Away from Instability.

Rivers R, Meininger C Int J Mol Sci. 2023; 24(6).

PMID: 36982254 PMC: 10048965. DOI: 10.3390/ijms24065181.


CO pneumoperitoneum effects on proliferation and apoptosis in two different neuroblastoma cell lines.

Curro M, Arena S, Montalto A, Perrone P, Di Fabrizio D, Bertuccio M Pediatr Surg Int. 2022; 38(3):457-464.

PMID: 35083505 DOI: 10.1007/s00383-022-05063-9.


Myricetin reduces cytotoxicity by suppressing hepcidin expression in MES23.5 cells.

Deng H, Liu S, Pan D, Jia Y, Ma Z Neural Regen Res. 2020; 16(6):1105-1110.

PMID: 33269757 PMC: 8224113. DOI: 10.4103/1673-5374.300461.


Hypoxia-Dependent Expression of TG2 Isoforms in Neuroblastoma Cells as Consequence of Different MYCN Amplification Status.

Curro M, Ferlazzo N, Giunta M, Montalto A, Russo T, Arena S Int J Mol Sci. 2020; 21(4).

PMID: 32085516 PMC: 7072980. DOI: 10.3390/ijms21041364.

References
1.
Marengo B, Raffaghello L, Pistoia V, Cottalasso D, Pronzato M, Marinari U . Reactive oxygen species: biological stimuli of neuroblastoma cell response. Cancer Lett. 2005; 228(1-2):111-6. DOI: 10.1016/j.canlet.2005.01.046. View

2.
Condello S, Curro M, Ferlazzo N, Caccamo D, Satriano J, Ientile R . Agmatine effects on mitochondrial membrane potential and NF-κB activation protect against rotenone-induced cell damage in human neuronal-like SH-SY5Y cells. J Neurochem. 2010; 116(1):67-75. DOI: 10.1111/j.1471-4159.2010.07085.x. View

3.
Montalto A, Bitto A, Irrera N, Polito F, Rinaldi M, Antonuccio P . CO2 pneumoperitoneum impact on early liver and lung cytokine expression in a rat model of abdominal sepsis. Surg Endosc. 2011; 26(4):984-9. DOI: 10.1007/s00464-011-1982-9. View

4.
Shen M, Feng Y, Gao C, Tao D, Hu J, Reed E . Detection of cyclin b1 expression in g(1)-phase cancer cell lines and cancer tissues by postsorting Western blot analysis. Cancer Res. 2004; 64(5):1607-10. DOI: 10.1158/0008-5472.can-03-3321. View

5.
Arsalani-Zadeh R, Ullah S, Khan S, MacFie J . Oxidative stress in laparoscopic versus open abdominal surgery: a systematic review. J Surg Res. 2011; 169(1):e59-68. DOI: 10.1016/j.jss.2011.01.038. View