Ultrabright Photoactivatable Fluorophores Created by Reductive Caging

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2012 Oct 30

PMID 23103881

Citations 78

Authors

Joshua C Vaughan

Shu Jia

Xiaowei Zhuang

Affiliations

Soon will be listed here.

Abstract

Sub-diffraction-limit imaging can be achieved by sequential localization of photoactivatable fluorophores, for which the image resolution depends on the number of photons detected per localization. We report a strategy for fluorophore caging that creates photoactivatable probes with high photon yields. Upon photoactivation, these probes can provide 10(4)-10(6) photons per localization and allow imaging of fixed samples with resolutions of several nanometers. This strategy can be applied to many fluorophores across the visible spectrum.

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