Experimental Infection of United States Swine with a Chinese Highly Pathogenic Strain of Porcine Reproductive and Respiratory Syndrome Virus

Overview

Journal Virology

Specialty Microbiology

Date 2012 Oct 20

PMID 23079105

Citations 50

Authors

Baoqing Guo

Kelly M Lager

Jamie N Henningson

Laura C Miller

Sarah N Schlink

Matthew A Kappes

Marcus E Kehrli Jr

Susan L Brockmeier

Tracy L Nicholson

Han-Chun Yang

Kay S Faaberg

Affiliations

Soon will be listed here.

Abstract

The pathogenesis of Type 2 highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) in 10-week old swine in the United States was investigated. rJXwn06, rescued from an infectious clone of Chinese HP-PRRSV, replicated in swine with at least 100-fold increased kinetics over U.S. strain VR-2332. rJXwn06 caused significant weight loss, exacerbated disease due to bacterial sepsis and more severe histopathological lung lesions in pigs exposed to HP-PRRSV than to those infected with VR-2332. Novel findings include identification of bacterial species present, the degree of thymic atrophy seen, and the inclusion of contact animals that highlighted the ability of HP-PRRSV to rapidly transmit between animals. Furthermore, comprehensive detailed cytokine analysis of serum, bronchoalveolar lavage fluid, and tracheobronchial lymph node tissue homogenate revealed a striking elevation in levels of cytokines associated with both innate and adaptive immunity in HP-PRRSV infected swine, and showed that contact swine differed in the degree of cytokine response.

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