Cell Sheet Technology for Regeneration of Esophageal Mucosa

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2012 Oct 16

PMID 23066307

Citations 21

Authors

Ryo Takagi

Masayuki Yamato

Nobuo Kanai

Daisuke Murakami

Makoto Kondo

Takaaki Ishii

Takeshi Ohki

Hideo Namiki

Masakazu Yamamoto

Teruo Okano

Affiliations

Soon will be listed here.

Abstract

The progress of tissue-engineering technology has realized development of new therapies to treat various disorders by using cultured cells. Cell- and tissue-based therapies have been successfully applied to human patients, and several tissue-engineered products have been approved by the regulatory agencies and are commercially available. In the review article, we describe our experience of development and clinical application of cell sheet-based regenerative medicine. Endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD) have been shown to be useful for removal of gastrointestinal neoplasms with less invasiveness compared with open surgery, especially in esophageal surgery. However, postoperative inflammation and stenosis are major complications observed after intensive mucosal resection. Therefore, we have developed novel regenerative medicine to prevent such complications and promote wound healing of esophageal mucosa after EMR or ESD. Transplantable oral mucosal epithelial cell sheets were fabricated from patients' own oral mucosa. Immediately after EMR or ESD, fabricated autologous cell sheets were endoscopically transplanted to the ulcer sites. We performed a preclinical study with a canine model. In human clinical settings, cell culture and cell sheet fabrication were performed in clean rooms according to good manufacturing practice guidelines, and pharmaceutical drugs were used as supplements to culture medium in place of research regents used in animal study. We believe that cell-based regenerative medicine would be useful to improve quality of life of patients after EMR or ESD.

Citing Articles

Simple and Cost-Effective Generation of 3D Cell Sheets and Spheroids Using Curvature-Controlled Paraffin Wax Substrates.

Kim H, Koo K, Kim C, Byun M, Park C, Son H Nano Converg. 2024; 11(1):44.

PMID: 39482392 PMC: 11527855. DOI: 10.1186/s40580-024-00451-4.

Single-cell Technology in Stem Cell Research.

Golchin A, Shams F, Moradi F, Sadrabadi A, Parviz S, Alipour S Curr Stem Cell Res Ther. 2024; 20(1):9-32.

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In-Vitro Decidualization With Different Progesterone Concentration: Development of a Hormone-Responsive 3D Endometrial Tissue Using Rat Endometrial Tissues.

Toma C, Kuramoto G, Homma J, Sakaguchi K, Shimizu T Cureus. 2023; 15(11):e49613.

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Recent Advances in Cell Sheet Engineering: From Fabrication to Clinical Translation.

Thummarati P, Laiwattanapaisal W, Nitta R, Fukuda M, Hassametto A, Kino-Oka M Bioengineering (Basel). 2023; 10(2).

PMID: 36829705 PMC: 9952256. DOI: 10.3390/bioengineering10020211.

Scalable fabrication, compartmentalization and applications of living microtissues.

Schot M, Araujo-Gomes N, van Loo B, Kamperman T, Leijten J Bioact Mater. 2022; 19:392-405.

PMID: 35574053 PMC: 9062422. DOI: 10.1016/j.bioactmat.2022.04.005.

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