Stimulation of Escherichia Coli DNA Damage Inducible DNA Helicase DinG by the Single-stranded DNA Binding Protein SSB

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2012 Oct 6

PMID 23036643

Citations 15

Authors

Zishuo Cheng

Aimee Caillet

Binbin Ren

Huangen Ding

Affiliations

Soon will be listed here.

Abstract

Escherichia coli DNA damage inducible protein DinG is a superfamily II DNA helicase and is closely related to human DNA helicase XPD. Here, we report that E. coli single-stranded DNA binding protein (SSB) is able to form a stable protein complex with DinG and to stimulate the DinG DNA helicase activity. An SSB mutant that retains the single-stranded DNA binding activity but fails to form a protein complex with DinG becomes a potent inhibitor for the DinG DNA helicase, suggesting that E. coli wild-type SSB stimulates the DinG DNA helicase via specific protein-protein interaction.

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