Activation-induced Cytidine Deaminase Alters the Subcellular Localization of Tet Family Proteins

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Oct 3

PMID 23028748

Citations 22

Authors

Yuko Arioka

Akira Watanabe

Kuniaki Saito

Yasuhiro Yamada

Affiliations

Soon will be listed here.

Abstract

Activation-induced cytidine deminase (Aid), a unique enzyme that deaminates cytosine in DNA, shuttles between the nucleus and the cytoplasm. A recent study proposed a novel function of Aid in active DNA demethylation via deamination of 5-hydroxymethylcytosine, which is converted from 5-methylcytosine by the Ten-eleven translocation (Tet) family of enzymes. In this study, we examined the effect of simultaneous expression of Aid and Tet family proteins on the subcellular localization of each protein. We found that overexpressed Aid is mainly localized in the cytoplasm, whereas Tet1 and Tet2 are localized in the nucleus, and Tet3 is localized in both the cytoplasm and the nucleus. However, nuclear Tet proteins were gradually translocated to the cytoplasm when co-expressed with Aid. We also show that Aid-mediated translocation of Tet proteins is associated with Aid shuttling. Here we propose a possible role for Aid as a regulator of the subcellular localization of Tet family proteins.

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