The Apolipoprotein B MRNA Editing Complex Performs a Multifunctional Cycle and Suppresses Nonsense-mediated Decay

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2003 Jul 26

PMID 12881431

Citations 60

Authors

Ann Chester

Angelika Somasekaram

Maria Tzimina

Adam Jarmuz

Jane Gisbourne

Raymond OKeefe

James Scott

Naveenan Navaratnam

Affiliations

Soon will be listed here.

Abstract

The C to U editing of apolipoprotein B (apoB) mRNA is mediated by a minimal complex composed of an RNA-binding cytidine deaminase (APOBEC1) and a complementing specificity factor (ACF). This editing generates a premature termination codon and a truncated open reading frame. We demonstrate that the APOBEC1-ACF holoenzyme mediates a multifunctional cycle. The atypical APOBEC1 nuclear localization signal is involved in RNA binding and is used to import ACF into the nucleus as cargo. APOBEC1 alone induces nonsense-mediated decay (NMD). The APOBEC1-ACF complex edits and remains associated with the edited RNA to protect it from NMD. The APOBEC1 nuclear export signal is involved in the export of ACF and the edited apoB mRNA together, to the site of translation.

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