HSL-knockout Mouse Testis Exhibits Class B Scavenger Receptor Upregulation and Disrupted Lipid Raft Microdomains

Overview

Journal J Lipid Res

Publisher Elsevier

Specialty Biochemistry

Date 2012 Sep 19

PMID 22988039

Citations 10

Authors

Maria Emilia Casado

Lydia Huerta

Ana Isabel Ortiz

Mirian Perez-Crespo

Alfonso Gutierrez-Adan

Fredric B Kraemer

Miguel Angel Lasuncion

Rebeca Busto

Antonia Martin-Hidalgo

Affiliations

Soon will be listed here.

Abstract

There is a tight relationship between fertility and changes in cholesterol metabolism during spermatogenesis. In the testis, class B scavenger receptors (SR-B) SR-BI, SR-BII, and LIMP II mediate the selective uptake of cholesterol esters from HDL, which are hydrolyzed to unesterified cholesterol by hormone-sensitive lipase (HSL). HSL is critical because HSL knockout (KO) male mice are sterile. The aim of the present work was to determine the effects of the lack of HSL in testis on the expression of SR-B, lipid raft composition, and related cell signaling pathways. HSL-KO mouse testis presented altered spermatogenesis associated with decreased sperm counts, sperm motility, and infertility. In wild-type (WT) testis, HSL is expressed in elongated spermatids; SR-BI, in Leydig cells and spermatids; SR-BII, in spermatocytes and spermatids but not in Leydig cells; and LIMP II, in Sertoli and Leydig cells. HSL knockout male mice have increased expression of class B scavenger receptors, disrupted caveolin-1 localization in lipid raft plasma membrane microdomains, and activated phospho-ERK, phospho-AKT, and phospho-SRC in the testis, suggesting that class B scavenger receptors are involved in cholesterol ester uptake for steroidogenesis and spermatogenesis in the testis.

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