Expression of FSH and Its Co-localization with FSH Receptor and GnRH Receptor in Rat Cerebellar Cortex

Overview

Journal J Mol Histol

Specialty Biochemistry

Date 2012 Sep 14

PMID 22972435

Citations 5

Authors

Chenyu Chu

Jingan Zhou

Yaqun Zhao

Ce Liu

Pengfei Chang

Qing Zhou

Li Zhao

Weiquan Huang

Affiliations

Soon will be listed here.

Abstract

The expression of follicle-stimulating hormone (FSH) and its receptor in extrapituitary and non-HPG axis tissues has been demonstrated and their non-reproductive functions in these tissues have been found. However, there have been no reports concerning the expression and function of FSH and its receptor in the cerebellum. In our study, immunofluorescence staining and in situ hybridization were used to detect the expression of FSH, double-labeled immunofluorescence staining was used to detect co-localization of FSH and its receptor and co-localization of FSH and gonadotropin-releasing hormone (GnRH) receptor in the rat cerebellar cortex. Results showed that some cells of the Purkinje cell layer, granular layer, and molecular layer of the cerebellar cortex showed both FSH immunoreactivity and FSH mRNA positive signals; not only for FSH and FSH receptor, but also for FSH and GnRH receptor co-localized in some cells throughout the Purkinje cell layer, granular layer, and molecular layer of the cerebellar cortex. These suggested that rat cerebellum could express FSH; cerebellum is a target tissue of FSH; FSH may exert certain functions through FSH receptor in a paracrine or autocrine manner; GnRH may regulate FSH positive cells through GnRH receptor in the cerebellum. Our study provides morphological evidence for further functional research on FSH and related hormones in the cerebellum.

Citing Articles

Characterization of reproductive hormones and related gene expression in the hypothalamus and pituitary gland in the egg-laying interval in White King pigeon.

Miao D, Liu C, Deng Z, Zhang C, Guo Z, Li W Poult Sci. 2024; 103(3):103422.

PMID: 38228063 PMC: 10823133. DOI: 10.1016/j.psj.2024.103422.

Gonadotropin-releasing hormone and growth hormone act as anti-inflammatory factors improving sensory recovery in female rats with thoracic spinal cord injury.

Martinez-Moreno C, Calderon-Vallejo D, Diaz-Galindo C, Hernandez-Jasso I, Olivares-Hernandez J, Avila-Mendoza J Front Neurosci. 2023; 17:1164044.

PMID: 37360158 PMC: 10288327. DOI: 10.3389/fnins.2023.1164044.

Altered Brain Structure and Functional Connectivity Associated with Pubertal Hormones in Girls with Precocious Puberty.

Chen T, Lu Y, Wang Y, Guo A, Xie X, Fu Y Neural Plast. 2020; 2019:1465632.

PMID: 31933625 PMC: 6942889. DOI: 10.1155/2019/1465632.

Growth Hormone (GH) and Gonadotropin-Releasing Hormone (GnRH) in the Central Nervous System: A Potential Neurological Combinatory Therapy?.

Martinez-Moreno C, Calderon-Vallejo D, Harvey S, Aramburo C, Quintanar J Int J Mol Sci. 2018; 19(2).

PMID: 29373545 PMC: 5855597. DOI: 10.3390/ijms19020375.

Knowledge-based compact disease models identify new molecular players contributing to early-stage Alzheimer's disease.

Mayburd A, Baranova A BMC Syst Biol. 2013; 7:121.

PMID: 24196233 PMC: 3827844. DOI: 10.1186/1752-0509-7-121.

References

Mizrachi D, Shemesh M . Follicle-stimulating hormone receptor and its messenger ribonucleic acid are present in the bovine cervix and can regulate cervical prostanoid synthesis. Biol Reprod. 1999; 61(3):776-84. DOI: 10.1095/biolreprod61.3.776. View

Andreescu C, Milojkovic B, Haasdijk E, Kramer P, de Jong F, Krust A . Estradiol improves cerebellar memory formation by activating estrogen receptor beta. J Neurosci. 2007; 27(40):10832-9. PMC: 6672828. DOI: 10.1523/JNEUROSCI.2588-07.2007. View

Zheng W, Magid M, KRAMER E, Chen Y . Follicle-stimulating hormone receptor is expressed in human ovarian surface epithelium and fallopian tube. Am J Pathol. 1996; 148(1):47-53. PMC: 1861603. View

Garde S, SHETH A, Joseph R, Panchal C, Chinoy R, SHETH N . Occurrence and de novo biosynthesis of follicle stimulating hormone (FSH) in benign and malignant conditions of human breast. Cancer Lett. 1993; 75(1):1-9. DOI: 10.1016/0304-3835(93)90200-s. View

Fujii S, Sato S, Fukui A, Kimura H, Kasai G, Saito Y . Continuous administration of gonadotrophin-releasing hormone agonist during the luteal phase in IVF. Hum Reprod. 2001; 16(8):1671-5. DOI: 10.1093/humrep/16.8.1671. View

Tojo K, Ichinose M, Nakayama M, Yamamoto H, Hasegawa T, Kawaguchi Y . A new family of Boucher-Neuhäuser syndrome: coexistence of Holmes type cerebellar atrophy, hypogonadotropic hypogonadism and retinochoroidal degeneration: case reports and review of literature. Endocr J. 1995; 42(3):367-76. DOI: 10.1507/endocrj.42.367. View

MacKenzie-Graham A, Rinek G, Avedisian A, Morales L, Umeda E, Boulat B . Estrogen treatment prevents gray matter atrophy in experimental autoimmune encephalomyelitis. J Neurosci Res. 2012; 90(7):1310-23. PMC: 3350614. DOI: 10.1002/jnr.23019. View

Coss D, Jacobs S, Bender C, Mellon P . A novel AP-1 site is critical for maximal induction of the follicle-stimulating hormone beta gene by gonadotropin-releasing hormone. J Biol Chem. 2003; 279(1):152-62. PMC: 2930619. DOI: 10.1074/jbc.M304697200. View

Gromoll J, Pekel E, Nieschlag E . The structure and organization of the human follicle-stimulating hormone receptor (FSHR) gene. Genomics. 1996; 35(2):308-11. DOI: 10.1006/geno.1996.0361. View

10.

You S, Kim H, Hsu C, El Halawani M, Foster D . Three different turkey luteinizing hormone receptor (tLH-R) isoforms I: characterization of alternatively spliced tLH-R isoforms and their regulated expression in diverse tissues. Biol Reprod. 1999; 62(1):108-16. DOI: 10.1095/biolreprod62.1.108. View

11.

Chu C, Xu B, Huang W . A study on expression of FSH and its effects on the secretion of insulin and glucagon in rat pancreas. Tissue Cell. 2010; 42(6):370-5. DOI: 10.1016/j.tice.2010.09.001. View

12.

Brioude F, Bouligand J, Trabado S, Francou B, Salenave S, Kamenicky P . Non-syndromic congenital hypogonadotropic hypogonadism: clinical presentation and genotype-phenotype relationships. Eur J Endocrinol. 2010; 162(5):835-51. DOI: 10.1530/EJE-10-0083. View

13.

Albertson A, Talbott H, Wang Q, Jensen D, Skinner D . The gonadotropin-releasing hormone type I receptor is expressed in the mouse cerebellum. Cerebellum. 2008; 7(3):379-84. DOI: 10.1007/s12311-008-0038-8. View

14.

Welle M, Reichler I, Barth A, Forster U, Sattler U, Arnold S . Immunohistochemical localization and quantitative assessment of GnRH-, FSH-, and LH-receptor mRNA Expression in canine skin: a powerful tool to study the pathogenesis of side effects after spaying. Histochem Cell Biol. 2006; 126(5):527-35. DOI: 10.1007/s00418-006-0189-y. View

15.

Findlay , DRUMMOND . Regulation of the FSH Receptor in the Ovary. Trends Endocrinol Metab. 2001; 10(5):183-188. DOI: 10.1016/s1043-2760(98)00144-1. View

16.

Mubaidin A, Till M, El-Shanti H, Hadidi A, Ajlouni K . Hypogonadotrophic hypogonadism, short stature, cerebellar ataxia, rod-cone retinal dystrophy, and hypersegmented neutrophils: a novel disorder or a new variant of Boucher-Neuhauser syndrome?. J Med Genet. 2003; 40(1):e2. PMC: 1735249. DOI: 10.1136/jmg.40.1.e2. View

17.

Tam J, Danilovich N, Nilsson K, Sairam M, Maysinger D . Chronic estrogen deficiency leads to molecular aberrations related to neurodegenerative changes in follitropin receptor knockout female mice. Neuroscience. 2002; 114(2):493-506. DOI: 10.1016/s0306-4522(02)00278-6. View

18.

Prat A, Behrendt M, Marcinkiewicz E, Boridy S, Sairam R, Seidah N . A novel mouse model of Alzheimer's disease with chronic estrogen deficiency leads to glial cell activation and hypertrophy. J Aging Res. 2011; 2011:251517. PMC: 3182380. DOI: 10.4061/2011/251517. View

19.

Maugars G, Schmitz M . Molecular cloning and characterization of FSH and LH receptors in Atlantic salmon (Salmo salar L.). Gen Comp Endocrinol. 2006; 149(1):108-17. DOI: 10.1016/j.ygcen.2006.04.011. View

20.

Fox H . Immunohistochemical localization of follicle-stimulating hormone, luteinizing hormone, growth hormone, adrenocorticotrophic hormone and prolactin in the human placenta. Placenta. 1986; 7(2):163-72. DOI: 10.1016/s0143-4004(86)80007-8. View