Characterization of Reproductive Hormones and Related Gene Expression in the Hypothalamus and Pituitary Gland in the Egg-laying Interval in White King Pigeon

Overview

Journal Poult Sci

Publisher Elsevier

Specialty Veterinary Medicine

Date 2024 Jan 16

PMID 38228063

Authors

D Z Miao

C Liu

Z Y Deng

C Zhang

Z Y Guo

W Q Li

Y Wang

H M Yang

Z Y Wang

Affiliations

Soon will be listed here.

Abstract

The egg-laying interval (LI) directly reflects the laying performance of breeding pigeons, influenced by reproductive hormones. This study aimed to assess reproductive hormone levels in serum and the expression of related genes and their receptors in the hypothalamus and pituitary gland in 4 stages: first (LI1), third (LI3), fifth (LI5), and seventh (LI7) days. The results showed that serum gonadotropin-releasing hormone (GnRH) level decreased from LI1 to LI7 (P < 0.01) and peaked in LI1. The serum follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels stayed at high levels from LI1 to LI5. The FSH level decreased slightly from LI5 to LI7 (P > 0.05), but the LH level decreased rapidly (P < 0.01). The prolactin (PRL) levels significantly increased in LI5 (P < 0.01) compared with LI1 and then stayed at a high level. The GnRH1 expression in the hypothalamus had no significant change in LI (P > 0.05). However, the GnRHR first decreased from LI1 to LI3 (P < 0.05) and then increased. The FSH mRNA level in the pituitary gland decreased from LI1 to LI3 and slightly increased in LI5 (P > 0.05). The change pattern of FSHR was similar to that of FSH and peaked in LI5 (P < 0.05). The LH expression level was the highest in LI5 and significantly higher than that in LI3 and LI7 (P < 0.05). However, the LHR mRNA level decreased in LI (P < 0.05). The expression patterns of PRL and PRLR were similar; they were upregulated in LI and peaked in LI7 (P < 0.01). The expression pattern of GnRHR was similar to that of FSH, LH, and FSHR, suggesting the critical role of GnRHR in LI. Furthermore, the expression levels of these genes peaked in LI5, closely correlating with the maturation of the first largest follicle in pigeons. PRL-PRLR signaling inhibited GnRH activity to promote ovulation. This study provided a basis for further investigating the molecular mechanisms underlying the regulation of reproduction in pigeons.

Citing Articles

Histological Characteristics of Follicles, Reproductive Hormones and Transcriptomic Analysis of White King Pigeon Illuminated with Red Light.

Wang Y, Zuo K, Zhang C, Miao D, Chen J, Yang H Animals (Basel). 2024; 14(16).

PMID: 39199854 PMC: 11350841. DOI: 10.3390/ani14162320.

References

Zhu H, Liu X, Hu M, Lei M, Chen Z, Ying S . Endocrine and molecular regulation mechanisms of the reproductive system of Hungarian White geese investigated under two artificial photoperiodic programs. Theriogenology. 2018; 123:167-176. DOI: 10.1016/j.theriogenology.2018.10.001. View

Huang Y, Shi Z, Liu Z, Liu Y, Li X . Endocrine regulations of reproductive seasonality, follicular development and incubation in Magang geese. Anim Reprod Sci. 2007; 104(2-4):344-58. DOI: 10.1016/j.anireprosci.2007.02.005. View

Chun S, Eisenhauer K, Minami S, Billig H, Perlas E, Hsueh A . Hormonal regulation of apoptosis in early antral follicles: follicle-stimulating hormone as a major survival factor. Endocrinology. 1996; 137(4):1447-56. DOI: 10.1210/endo.137.4.8625923. View

Sonez M, Sonez C, Mugnaini M, Haedo M, Romera S, Lombardo D . Effects of differential pulse frequencies of chicken gonadotrophin-releasing hormone-I (cGnRH-I) on laying hen gonadotrope responses in vitro. Biotech Histochem. 2009; 85(6):355-63. DOI: 10.3109/10520290903368774. View

Wang Y, Yang H, Zi C, Gu J, Wang Z . The mediation of pigeon egg production by regulating the steroid hormone biosynthesis of pigeon ovarian granulosa cells. Poult Sci. 2020; 99(11):6075-6083. PMC: 7647703. DOI: 10.1016/j.psj.2020.06.048. View

Ghanem K, Johnson A . Follicle dynamics and granulosa cell differentiation in the turkey hen ovary. Poult Sci. 2018; 97(10):3755-3761. DOI: 10.3382/ps/pey224. View

Scanes C, Sharp P, Chadwick A . Changes in plasma prolactin concentration during the ovulatory cycle of the chicken. J Endocrinol. 1977; 72(3):401-2. DOI: 10.1677/joe.0.0720401. View

Peczely P, El Halawani M, Hargitai C, Mezes M, Forgo V, Janosi S . The photorefractoriness in domestic goose: effect of gonads and thyroid on the development of postbreeding prolactinemia. Acta Biol Hung. 1993; 44(4):329-52. View

Zhang Y, Chen Z, An C, Weng K, Cao Z, Xu Q . Effect of active immunization with recombinant-derived goose INH-α, AMH, and PRL fusion protein on broodiness onset and egg production in geese (Anser cygnoides). Poult Sci. 2021; 100(11):101452. PMC: 8531861. DOI: 10.1016/j.psj.2021.101452. View

10.

Allan C, Wang Y, Jimenez M, Marshan B, Spaliviero J, Illingworth P . Follicle-stimulating hormone increases primordial follicle reserve in mature female hypogonadal mice. J Endocrinol. 2006; 188(3):549-57. DOI: 10.1677/joe.1.06614. View

11.

Tan Y, Xu X, Cao H, Zhou W, Yin Z . Effect of age at first egg on reproduction performance and characterization of the hypothalamo-pituitary-gonadal axis in chickens. Poult Sci. 2021; 100(9):101325. PMC: 8313835. DOI: 10.1016/j.psj.2021.101325. View

12.

Li C, Cao Y, Ren Y, Zhao Y, Wu X, Si S . The adiponectin receptor agonist, AdipoRon, promotes reproductive hormone secretion and gonadal development via the hypothalamic-pituitary-gonadal axis in chickens. Poult Sci. 2022; 102(2):102319. PMC: 9763694. DOI: 10.1016/j.psj.2022.102319. View

13.

Mellouk N, Rame C, Barbe A, Grandhaye J, Froment P, Dupont J . Chicken Is a Useful Model to Investigate the Role of Adipokines in Metabolic and Reproductive Diseases. Int J Endocrinol. 2018; 2018:4579734. PMC: 6029501. DOI: 10.1155/2018/4579734. View

14.

Brady K, Porter T, Liu H, Long J . Characterization of the hypothalamo-pituitary-gonadal axis in low and high egg producing turkey hens. Poult Sci. 2020; 99(2):1163-1173. PMC: 7587793. DOI: 10.1016/j.psj.2019.12.028. View

15.

Zhang Y, Wang S, Huang X, Li K, Chen W, Ruan D . Estimation of dietary manganese requirement for laying duck breeders: effects on productive and reproductive performance, egg quality, tibial characteristics, and serum biochemical and antioxidant indices. Poult Sci. 2020; 99(11):5752-5762. PMC: 7647759. DOI: 10.1016/j.psj.2020.06.076. View

16.

Wang Y, Guo Z, Zhang C, Miao D, Mao X, Lu S . Characterization of ovarian follicles, serum steroid hormone concentration, and steroidogenic gene expression profiles in the developing ovarian follicles in White King pigeons. Poult Sci. 2023; 102(7):102673. PMC: 10173770. DOI: 10.1016/j.psj.2023.102673. View

17.

Yang P, Medan M, Arai K, Watanabe G, Taya K . Plasma concentrations of immunoreactive (ir)-inhibin, gonadotropins and steroid hormones during the ovulatory cycle of the duck. J Reprod Dev. 2005; 51(3):353-8. DOI: 10.1262/jrd.16093. View

18.

Wan B, Yuan X, Yang W, Jiao N, Li Y, Liu F . The Effects of Zearalenone on the Localization and Expression of Reproductive Hormones in the Ovaries of Weaned Gilts. Toxins (Basel). 2021; 13(9). PMC: 8470812. DOI: 10.3390/toxins13090626. View

19.

Yang M, Lin Y, Meng F, Du X, Zeng X, Bu G . Characterization of prolactin (PRL) and PRL receptor (PRLR) in Chinese soft-shelled turtle: Molecular identification, ligand-receptor interaction and tissue distribution. Gen Comp Endocrinol. 2021; 316:113941. DOI: 10.1016/j.ygcen.2021.113941. View

20.

Kokoszynski D, Steczny K, Zochowska-Kujawska J, Sobczak M, Kotowicz M, Saleh M . Carcass Characteristics, Physicochemical Properties, and Texture and Microstructure of the Meat and Internal Organs of Carrier and King Pigeons. Animals (Basel). 2020; 10(8). PMC: 7459732. DOI: 10.3390/ani10081315. View