MicroRNA-99b Acts As a Tumor Suppressor in Non-small Cell Lung Cancer by Directly Targeting Fibroblast Growth Factor Receptor 3

Overview

Journal Exp Ther Med

Specialty Pathology

Date 2012 Sep 13

PMID 22969861

Citations 31

Authors

Jaeku Kang

Soo Young Lee

Sun Young Lee

Young Jin Kim

Jae Yong Park

Sun Jung Kwon

Moon Jun Na

Eun Jin Lee

Hyo Sung Jeon

Ji Woong Son

Affiliations

Soon will be listed here.

Abstract

microRNAs (miRNAs) play a significant role in cancer development and progression by regulating the expression of proto-oncogenes or tumor suppressor genes. Our previous study using microarrays demonstrated that miR-99b was downregulated in patients with lung cancer. To assess whether or not miR-99b has a functional role in lung cancer, we determined the expression of miR-99b and fibroblast growth factor receptor 3 (FGFR3), which is a predicted target of miR-99b in public algorithms in human lung cancer tissues. miR-99b was downregulated and FGFR3 was upregulated in lung cancer patients. We demonstrated that the overexpression of miR-99b induced a reduction in FGFR3 expression and confirmed the target specificity between miR-99b and the FGFR3 3'-untranslated region by luciferase reporter assay. In addition, the growth rate in miR-99b precursor-treated cells was lower compared to the negative controls. Taken together, these results suggest that miR-99b may be a tumor suppressor through the downregulation of FGFR3. miR-99b may be a potent tumor suppressor and may be a potential therapeutic tool for patients with lung cancer.

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