Effect of Cigarette Smoke Extract on Insulin Transport in Alveolar Epithelial Cell Line A549

Overview

Journal Lung

Specialty Pulmonary Medicine

Date 2012 Sep 11

PMID 22960792

Citations 4

Authors

Mikihisa Takano

Taishi Horiuchi

Junya Nagai

Ryoko Yumoto

Affiliations

Soon will be listed here.

Abstract

Background: The main purpose of this study was to evaluate the effect of cigarette smoke extract (CSE) on insulin transport in alveolar epithelial cells.

Methods: We first examined the effect of CSE pretreatment on cell viability, mRNA expression, and lamellar body structures in A549 cells. Then the effect of CSE pretreatment on FITC-insulin transport was examined.

Results: When A549 cells were treated with 30 μg/ml of CSE for 48 h, the expression of some mRNAs abundantly expressed in type II alveolar epithelial cells such as surfactant protein B was significantly increased. Lamellar bodylike structures became more evident with CSE treatment. FITC-insulin uptake from the apical side and subsequent efflux to the basal side was enhanced by CSE treatment in A549 cells. The enhancing effect of CSE on FITC-insulin uptake was concentration-dependent and reversible. A concentration-dependent enhancing effect of CSE on FITC-insulin uptake was also observed in normal, primary cultured alveolar type II epithelial cells isolated from rats.

Conclusions: Treatment of A549 cells by CSE may direct the cells to a more type II-like phenotype. In accordance with this observation, FITC-insulin uptake was enhanced by CSE treatment. These results may partly explain the higher insulin absorption from the lung in smokers than in nonsmokers.

Citing Articles

Molecular Impact of Conventional and Electronic Cigarettes on Pulmonary Surfactant.

Garavaglia M, Bodega F, Porta C, Milzani A, Sironi C, Dalle-Donne I Int J Mol Sci. 2023; 24(14).

PMID: 37511463 PMC: 10380520. DOI: 10.3390/ijms241411702.

A Review of the Impact of Smoking on Inhaled Insulin: Would You Stop Smoking if Insulin Can Be Inhaled?.

Malhotra P, Akku R, Jayaprakash T, Ogbue O, Khan S Cureus. 2020; 12(7):e9364.

PMID: 32850233 PMC: 7445000. DOI: 10.7759/cureus.9364.

Mechanisms and modulation of microvesicle uptake in a model of alveolar cell communication.

Schneider D, Speth J, Penke L, Wettlaufer S, Swanson J, Peters-Golden M J Biol Chem. 2017; 292(51):20897-20910.

PMID: 29101235 PMC: 5743066. DOI: 10.1074/jbc.M117.792416.

Functional Expression of PEPT2 in the Human Distal Lung Epithelial Cell Line NCl-H441.

Takano M, Sugimoto N, Ehrhardt C, Yumoto R Pharm Res. 2015; 32(12):3916-26.

PMID: 26168863 DOI: 10.1007/s11095-015-1751-x.

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