Comparison of Albumin Uptake in Rat Alveolar Type II and Type I-like Epithelial Cells in Primary Culture

Overview

Journal Pharm Res

Specialties Pharmacology
Pharmacy

Date 2007 Sep 14

PMID 17851738

Citations 21

Authors

Mika Ikehata

Ryoko Yumoto

Kosuke Nakamura

Junya Nagai

Mikihisa Takano

Affiliations

Soon will be listed here.

Abstract

Purpose: To elucidate and compare the activity and mechanism of albumin uptake in primary cultured alveolar type II and type I-like epithelial cells.

Materials And Methods: Type II epithelial cells isolated from rat lungs were cultured for 2 days at 5 x 10(6) cells/35-mm dish or for 6 days at 2 x 10(6) cells/35-mm dish. The mRNA expression of marker genes and FITC-albumin uptake were examined.

Results: The cells cultured for 2 days exhibited cuboidal type II epithelial morphology with lamellar bodies inside the cells, while the cells cultured for 6 days exhibited squamous type I epithelial morphology. These morphological characteristics were consistent with the changes in mRNA expression pattern of marker genes. FITC-albumin uptake in both cells was temperature-dependent and was inhibited by metabolic inhibitors and bafilomycin A1. The rate of uptake was much higher in type II cells than type I-like cells. In both cells, FITC-albumin uptake was inhibited by clathrin mediated-endocytosis inhibitors, but not by caveolae mediated-endocytosis inhibitors.

Conclusions: These findings indicate that albumin in alveolar lining fluid is internalized into type II and type I epithelial cells via clathrin-mediated endocytosis, and the rate of albumin uptake is higher in type II cells than type I cells.

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