» Articles » PMID: 2292596

Purification and Characterization of Soluble Human IL-6 Receptor Expressed in CHO Cells

Overview
Journal J Biochem
Specialty Biochemistry
Date 1990 Oct 1
PMID 2292596
Citations 34
Authors
Affiliations
Soon will be listed here.
Abstract

An immunosorbent assay system to detect genetically engineered IL-6 receptor (IL-6R) was established, whereby soluble IL-6 receptor (sIL-6R) was detected in the culture medium when sIL-6R cDNA was transfected into COS1 cells. A stably transformed Chinese hamster ovary (CHO) cell line constitutively expressing sIL-6R has been established. The recombinant sIL-6R was purified to homogeneity by sequential filtration and chromatography of the culture medium. The recombinant sIL-6R augmented the sensitivity of M1 cells to IL-6 in growth inhibition assay in a dose-dependent manner. Furthermore, a radioisotope immunosorbent assay (RIA) utilizing recombinant sIL-6R was established which could detect IL-6 in a quantity as low as 10 ng/ml.

Citing Articles

High Induction of IL-6 Secretion From hUCMSCs Optimize the Potential of hUCMSCs and TCZ as Therapy for COVID-19-Related ARDS.

Lo H, Cheng S, Huang J, Chang K, Chang Y, Huang C Cell Transplant. 2021; 30:9636897211054481.

PMID: 34757857 PMC: 8586187. DOI: 10.1177/09636897211054481.


The immunobiology of humanized Anti-IL6 receptor antibody: From basic research to breakthrough medicine.

Ohsugi Y J Transl Autoimmun. 2020; 3:100030.

PMID: 32743515 PMC: 7388389. DOI: 10.1016/j.jtauto.2019.100030.


Epistasis analysis links immune cascades and cerebral amyloidosis.

Benedet A, Labbe A, Lemay P, Zimmer E, Pascoal T, Leuzy A J Neuroinflammation. 2015; 12:227.

PMID: 26626881 PMC: 4666175. DOI: 10.1186/s12974-015-0436-z.


A multiscale model of interleukin-6-mediated immune regulation in Crohn's disease and its application in drug discovery and development.

Dwivedi G, Fitz L, Hegen M, Martin S, Harrold J, Heatherington A CPT Pharmacometrics Syst Pharmacol. 2014; 3:e89.

PMID: 24402116 PMC: 3910013. DOI: 10.1038/psp.2013.64.


Interleukin-6 contributes to CNS axon regeneration upon inflammatory stimulation.

Leibinger M, Muller A, Gobrecht P, Diekmann H, Andreadaki A, Fischer D Cell Death Dis. 2013; 4:e609.

PMID: 23618907 PMC: 3641349. DOI: 10.1038/cddis.2013.126.