Improvement of Cardiac Function and Reversal of Gap Junction Remodeling by Neuregulin-1β in Volume-overloaded Rats with Heart Failure

Overview

Journal J Geriatr Cardiol

Specialty Geriatrics

Date 2012 Aug 24

PMID 22916065

Citations 12

Authors

Xue-Hui Wang

Xiao-Zhen Zhuo

Ya-Juan Ni

Min Gong

Ting-Zhong Wang

Qun Lu

Ai-Qun Ma

Affiliations

Soon will be listed here.

Abstract

Objective: We performed experiments using Neuregulin-1β (NRG-1β) treatment to determine a mechanism for the protective role derived from its beneficial effects by remodeling gap junctions (GJs) during heart failure (HF).

Methods: Rat models of HF were established by aortocaval fistula. Forty-eight rats were divided randomly into the HF (HF, n = 16), NRG-1β treatment (NRG, n = 16), and sham operation (S, n = 16) group. The rats in the NRG group were administered NRG-1β (10 µg/kg per day) for 7 days via the tail vein, whereas the other groups were injected with the same doses of saline. Twelve weeks after operation, Connexin 43 (Cx43) expression in single myocytes obtained from the left ventricle was determined by immunocytochemistry. Total protein was extracted from frozen left ventricular tissues for immunoblotting assay, and the ultrastructure of myocytes was observed by transmission electron microscopy.

Results: Compared with the HF group, the cardiac function of rats in the NRG group was markedly improved, irregular distribution and deceased Cx43 expression were relieved. The ultrastructure of myocytes was seriously damaged in HF rats, and NRG-1β reduced these pathological damages.

Conclusions: Short-term NRG-1β treatment can rescue pump failure in experimental models of volume overload-induced HF, which is related to the recovery of GJs structure and the improvement of Cx43 expression.

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