MicroRNAs MiR-30b, MiR-30d, and MiR-494 Regulate Human Endometrial Receptivity

Overview

Journal Reprod Sci

Publisher Springer

Specialty Reproductive Medicine

Date 2012 Aug 21

PMID 22902743

Citations 76

Authors

Signe Altmae

Jose A Martinez-Conejero

Francisco J Esteban

Maria Ruiz-Alonso

Anneli Stavreus-Evers

Jose A Horcajadas

Andres Salumets

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) act as important epigenetic posttranscriptional regulators of gene expression. We aimed to gain more understanding of the complex gene expression regulation of endometrial receptivity by analyzing miRNA signatures of fertile human endometria. We set up to analyze miRNA signatures of receptive (LH + 7, n = 4) versus prereceptive (LH + 2, n = 5) endometrium from healthy fertile women. We found hsa-miR-30b and hsa-miR-30d to be significantly upregulated, and hsa-miR-494 and hsa-miR-923 to be downregulated in receptive endometrium. Three algorithms (miRanda, PicTar, and TargetScan) were used for target gene prediction. Functional analyses of the targets using Ingenuity Pathways Analysis and The Database for Annotation, Visualization and Integrated Discovery indicated roles in transcription, cell proliferation and apoptosis, and significant involvement in several relevant pathways, such as axon guidance, Wnt/β-catenin, ERK/MAPK, transforming growth factor β (TGF-β), p53 and leukocyte extravasation. Comparison of predicted miRNA target genes and our previous messenger RNA microarray data resulted in a list of 12 genes, including CAST, CFTR, FGFR2, and LIF that could serve as a panel of genes important for endometrial receptivity. In conclusion, we suggest that a subset of miRNAs and their target genes may play important roles in endometrial receptivity.

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References

Sha A, Liu J, Jiang X, Ren J, Ma C, Lei W . Genome-wide identification of micro-ribonucleic acids associated with human endometrial receptivity in natural and stimulated cycles by deep sequencing. Fertil Steril. 2011; 96(1):150-155.e5. DOI: 10.1016/j.fertnstert.2011.04.072. View

Aghajanova L, Hamilton A, Giudice L . Uterine receptivity to human embryonic implantation: histology, biomarkers, and transcriptomics. Semin Cell Dev Biol. 2007; 19(2):204-11. PMC: 2829661. DOI: 10.1016/j.semcdb.2007.10.008. View

Dickinson R, Duncan W . The SLIT-ROBO pathway: a regulator of cell function with implications for the reproductive system. Reproduction. 2010; 139(4):697-704. PMC: 2971463. DOI: 10.1530/REP-10-0017. View

Riesewijk A, Martin J, van Os R, Horcajadas J, Polman J, Pellicer A . Gene expression profiling of human endometrial receptivity on days LH+2 versus LH+7 by microarray technology. Mol Hum Reprod. 2003; 9(5):253-64. DOI: 10.1093/molehr/gag037. View

Zhang D, Lei C, Zhang W . Up-regulated monoamine oxidase in the mouse uterus during the peri-implantation period. Arch Gynecol Obstet. 2010; 284(4):861-6. DOI: 10.1007/s00404-010-1765-x. View

Michel V, Bakovic M . The solute carrier 44A1 is a mitochondrial protein and mediates choline transport. FASEB J. 2009; 23(8):2749-58. DOI: 10.1096/fj.08-121491. View

Genbacev O, Prakobphol A, Foulk R, Krtolica A, Ilic D, Singer M . Trophoblast L-selectin-mediated adhesion at the maternal-fetal interface. Science. 2003; 299(5605):405-8. DOI: 10.1126/science.1079546. View

Krek A, Grun D, Poy M, Wolf R, Rosenberg L, Epstein E . Combinatorial microRNA target predictions. Nat Genet. 2005; 37(5):495-500. DOI: 10.1038/ng1536. View

Boivin J, Bunting L, Collins J, Nygren K . International estimates of infertility prevalence and treatment-seeking: potential need and demand for infertility medical care. Hum Reprod. 2007; 22(6):1506-12. DOI: 10.1093/humrep/dem046. View

10.

Hu S, Ren G, Liu J, Zhao Z, Yu Y, Su R . MicroRNA expression and regulation in mouse uterus during embryo implantation. J Biol Chem. 2008; 283(34):23473-84. DOI: 10.1074/jbc.M800406200. View

11.

Li R, Qiao J, Wang L, Li L, Zhen X, Liu P . MicroRNA array and microarray evaluation of endometrial receptivity in patients with high serum progesterone levels on the day of hCG administration. Reprod Biol Endocrinol. 2011; 9:29. PMC: 3068947. DOI: 10.1186/1477-7827-9-29. View

12.

Lim L, Lau N, Garrett-Engele P, Grimson A, Schelter J, Castle J . Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature. 2005; 433(7027):769-73. DOI: 10.1038/nature03315. View

13.

Igci Y, Arslan A, Akarsu E, Erkilic S, Igci M, Oztuzcu S . Differential expression of a set of genes in follicular and classic variants of papillary thyroid carcinoma. Endocr Pathol. 2011; 22(2):86-96. DOI: 10.1007/s12022-011-9157-8. View

14.

Carthew R, Sontheimer E . Origins and Mechanisms of miRNAs and siRNAs. Cell. 2009; 136(4):642-55. PMC: 2675692. DOI: 10.1016/j.cell.2009.01.035. View

15.

Dominguez F, Yanez-Mo M, Sanchez-Madrid F, Simon C . Embryonic implantation and leukocyte transendothelial migration: different processes with similar players?. FASEB J. 2005; 19(9):1056-60. DOI: 10.1096/fj.05-3781hyp. View

16.

Egger G, Liang G, Aparicio A, Jones P . Epigenetics in human disease and prospects for epigenetic therapy. Nature. 2004; 429(6990):457-63. DOI: 10.1038/nature02625. View

17.

Friedman R, Farh K, Burge C, Bartel D . Most mammalian mRNAs are conserved targets of microRNAs. Genome Res. 2008; 19(1):92-105. PMC: 2612969. DOI: 10.1101/gr.082701.108. View

18.

Fernando R, Nettleton D, Southey B, Dekkers J, Rothschild M, Soller M . Controlling the proportion of false positives in multiple dependent tests. Genetics. 2004; 166(1):611-9. PMC: 1470704. DOI: 10.1534/genetics.166.1.611. View

19.

Noyes R, Hertig A, Rock J . Dating the endometrial biopsy. Am J Obstet Gynecol. 1975; 122(2):262-3. DOI: 10.1016/s0002-9378(16)33500-1. View

20.

Altmae S, Martinez-Conejero J, Salumets A, Simon C, Horcajadas J, Stavreus-Evers A . Endometrial gene expression analysis at the time of embryo implantation in women with unexplained infertility. Mol Hum Reprod. 2009; 16(3):178-87. DOI: 10.1093/molehr/gap102. View