MicroRNA Array and Microarray Evaluation of Endometrial Receptivity in Patients with High Serum Progesterone Levels on the Day of HCG Administration

Overview

Journal Reprod Biol Endocrinol

Publisher Biomed Central

Specialties Endocrinology
Reproductive Medicine

Date 2011 Mar 8

PMID 21375772

Citations 35

Authors

Rong Li

Jie Qiao

Lina Wang

Li Li

Xiumei Zhen

Ping Liu

Xiaoying Zheng

Affiliations

Soon will be listed here.

Abstract

Background: To determine the effect of higher progesterone (P) level on endometrial receptivity.

Methods: This was a prospective analysis conducted in the Reproductive Medical Center of Peking University Third Hospital. All patients received IVF treatment and canceled embryo transfer in the same cycle and were divided into group 1 (normal P; 7 patients) and group 2 (elevated P; 12 patients). Endometrial biopsies were performed 6 days after oocyte retrieval. The global miRNA and mRNA gene expressions in endometrial biopsies were investigated with a V4.0 miRNA probe and 22 K Human Genome Array. Fold ratios were derived to compare gene regulation between the groups. Spp1 and Ang gene expression was selected to verify the array results by RT-PCR and the protein expression of osteopontin and VEGF was determined using an immunohistochemical method.

Results: There were 4 miRNA (all down-regulated) and 22 mRNA (13 up-regulated and 9 down-regulated) exhibiting differential expression between the groups on the microRNA and microarray chips. miRNA-451, Spp1, and Ang expression in RT-PCR verified the array results. Osteopontin and VEGF were also shown to have positive expression in the endometrium.

Conclusions: Data from microRNA and microarray analysis suggests dissimilar endometrial receptivity in patients with high P levels on the day of hCG, and elevated osteopontin and decreased VEGF had poor pregnancy rates.

Citing Articles

MicroRNAs, endometrial receptivity and molecular pathways.

Salmasi S, Heidar M, Khaksary Mahabady M, Rashidi B, Mirzaei H Reprod Biol Endocrinol. 2024; 22(1):139.

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Altered microRNA composition in the uterine lumen fluid in cattle (Bos taurus) pregnancies initiated by artificial insemination or transfer of an in vitro produced embryo.

Biase F, Moorey S, Schnuelle J, Rodning S, Ortega M, Spencer T J Anim Sci Biotechnol. 2024; 15(1):130.

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hMG addition affects the change in progesterone level during IVF stimulation and LBR: a retrospective cohort study.

Wesevich V, Seckin S, Kelk D, Kallen A, Kodaman P Reprod Biol Endocrinol. 2023; 21(1):117.

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Azizi E, Mofarahe Z, Naji M J Assist Reprod Genet. 2023; 40(4):697-717.

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References

Varghese A, Goldberg E, Bhattacharyya A, Agarwal A . Emerging technologies for the molecular study of infertility, and potential clinical applications. Reprod Biomed Online. 2007; 15(4):451-6. DOI: 10.1016/s1472-6483(10)60372-0. View

Aplin J . Embryo implantation: the molecular mechanism remains elusive. Reprod Biomed Online. 2006; 13(6):833-9. DOI: 10.1016/s1472-6483(10)61032-2. View

Aghajanova L, Hamilton A, Giudice L . Uterine receptivity to human embryonic implantation: histology, biomarkers, and transcriptomics. Semin Cell Dev Biol. 2007; 19(2):204-11. PMC: 2829661. DOI: 10.1016/j.semcdb.2007.10.008. View

Giudice L, Telles T, Lobo S, Kao L . The molecular basis for implantation failure in endometriosis: on the road to discovery. Ann N Y Acad Sci. 2002; 955:252-64; discussion 293-5, 396-406. DOI: 10.1111/j.1749-6632.2002.tb02786.x. View

Talbi S, Hamilton A, Vo K, Tulac S, Overgaard M, Dosiou C . Molecular phenotyping of human endometrium distinguishes menstrual cycle phases and underlying biological processes in normo-ovulatory women. Endocrinology. 2005; 147(3):1097-121. DOI: 10.1210/en.2005-1076. View

Giudice L . Potential biochemical markers of uterine receptivity. Hum Reprod. 2000; 14 Suppl 2:3-16. DOI: 10.1093/humrep/14.suppl_2.3. View

Watanabe T, Takeda A, Mise K, Okuno T, Suzuki T, Minami N . Stage-specific expression of microRNAs during Xenopus development. FEBS Lett. 2005; 579(2):318-24. DOI: 10.1016/j.febslet.2004.11.067. View

Shulman A, Ghetler Y, Beyth Y . The significance of an early (premature) rise of plasma progesterone in in vitro fertilization cycles induced by a "long protocol" of gonadotropin releasing hormone analogue and human menopausal gonadotropins. J Assist Reprod Genet. 1996; 13(3):207-11. DOI: 10.1007/BF02065937. View

Sontheimer E, Carthew R . Silence from within: endogenous siRNAs and miRNAs. Cell. 2005; 122(1):9-12. DOI: 10.1016/j.cell.2005.06.030. View

10.

Fazleabas A, Donnelly K, Srinivasan S, Fortman J, Miller J . Modulation of the baboon (Papio anubis) uterine endometrium by chorionic gonadotrophin during the period of uterine receptivity. Proc Natl Acad Sci U S A. 1999; 96(5):2543-8. PMC: 26821. DOI: 10.1073/pnas.96.5.2543. View

11.

Pfaffl M . A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001; 29(9):e45. PMC: 55695. DOI: 10.1093/nar/29.9.e45. View

12.

Melo M, Meseguer M, Garrido N, Bosch E, Pellicer A, Remohi J . The significance of premature luteinization in an oocyte-donation programme. Hum Reprod. 2006; 21(6):1503-7. DOI: 10.1093/humrep/dei474. View

13.

Thomson J, Parker J, Perou C, Hammond S . A custom microarray platform for analysis of microRNA gene expression. Nat Methods. 2005; 1(1):47-53. DOI: 10.1038/nmeth704. View

14.

Tusher V, Tibshirani R, Chu G . Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci U S A. 2001; 98(9):5116-21. PMC: 33173. DOI: 10.1073/pnas.091062498. View

15.

Carson D, Lagow E, Thathiah A, Al-Shami R, Farach-Carson M, Vernon M . Changes in gene expression during the early to mid-luteal (receptive phase) transition in human endometrium detected by high-density microarray screening. Mol Hum Reprod. 2002; 8(9):871-9. DOI: 10.1093/molehr/8.9.871. View

16.

Nikas G, Develioglu O, Toner J, Jones Jr H . Endometrial pinopodes indicate a shift in the window of receptivity in IVF cycles. Hum Reprod. 1999; 14(3):787-92. DOI: 10.1093/humrep/14.3.787. View

17.

Li R, Qiao J, Wang L, Zhen X, Lu Y . Serum progesterone concentration on day of HCG administration and IVF outcome. Reprod Biomed Online. 2008; 16(5):627-31. DOI: 10.1016/s1472-6483(10)60475-0. View

18.

Salamonsen L . Role of proteases in implantation. Rev Reprod. 1999; 4(1):11-22. DOI: 10.1530/ror.0.0040011. View

19.

Fujimoto J, Ichigo S, Hori M, Tamaya T . Alteration of E-cadherin, alpha- and beta-catenin mRNA expression in human uterine endometrium during the menstrual cycle. Gynecol Endocrinol. 1996; 10(3):187-91. DOI: 10.3109/09513599609027987. View

20.

Doench J, Sharp P . Specificity of microRNA target selection in translational repression. Genes Dev. 2004; 18(5):504-11. PMC: 374233. DOI: 10.1101/gad.1184404. View