Regulation of DNA Replication Timing on Human Chromosome by a Cell-type Specific DNA Binding Protein SATB1

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Aug 11

PMID 22879953

Citations 4

Authors

Masako Oda

Yutaka Kanoh

Yoshihisa Watanabe

Hisao Masai

Affiliations

Soon will be listed here.

Abstract

Background: Replication timing of metazoan DNA during S-phase may be determined by many factors including chromosome structures, nuclear positioning, patterns of histone modifications, and transcriptional activity. It may be determined by Mb-domain structures, termed as "replication domains", and recent findings indicate that replication timing is under developmental and cell type-specific regulation.

Methodology/principal Findings: We examined replication timing on the human 5q23/31 3.5-Mb segment in T cells and non-T cells. We used two independent methods to determine replication timing. One is quantification of nascent replicating DNA in cell cycle-fractionated stage-specific S phase populations. The other is FISH analyses of replication foci. Although the locations of early- and late-replicating domains were common between the two cell lines, the timing transition region (TTR) between early and late domains were offset by 200-kb. We show that Special AT-rich sequence Binding protein 1 (SATB1), specifically expressed in T-cells, binds to the early domain immediately adjacent to TTR and delays the replication timing of the TTR. Measurement of the chromosome copy number along the TTR during synchronized S phase suggests that the fork movement may be slowed down by SATB1.

Conclusions: Our results reveal a novel role of SATB1 in cell type-specific regulation of replication timing along the chromosome.

Citing Articles

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Synchronized replication of genes encoding the same protein complex in fast-proliferating cells.

Chen Y, Li K, Chu X, Carey L, Qian W Genome Res. 2019; 29(12):1929-1938.

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Cell line differences in replication timing of human glutamate receptor genes and other large genes associated with neural disease.

Watanabe Y, Shibata K, Maekawa M Epigenetics. 2014; 9(10):1350-9.

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Developmental control of replication timing defines a new breed of chromosomal domains with a novel mechanism of chromatin unfolding.

Takebayashi S, Ryba T, Gilbert D Nucleus. 2012; 3(6):500-7.

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