DNA Synthesis in Temperature-sensitive Mutants of the Cell Cycle Infected by Polyoma Virus and Adenovirus

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1979 Sep 1

PMID 228298

Citations 6

Authors

M Rossini

R Weinmann

R Baserga

Affiliations

Soon will be listed here.

Abstract

tsAF8 cells are a temperature-sensitive (ts) mutant of BHK cells that are arrested in G1 at the nonpermissive temperature. When made quiescent by serum restriction, they can be stimulated to enter S phase by 10% serum at 34 degrees C but not at 40.6 degrees C. The same results can be obtained if quiescent cells are infected with polyoma virus or adenovirus 12 instead of serum. However, adenovirus 2 infection stimulates DNA synthesis in tsAF8 cells at both 34 degrees C and 40.6 degrees C. The DNA synthesized after adenovirus 2 infection has been shown to be cellular DNA by CsCl density centrifugation. By density labeling it can be shown that adenovirus 2-induced DNA synthesis is due to semiconservative replication. The difference between adenovirus 2 and polyoma (or serum) is also evident with another ts mutant of BHK cells, ts13 cells. These results open the possibility of identifying the viral or cellular mechanism at the basis of this difference in the induction of host DNA synthesis between adenovirus 2 and polyoma or serum.

Citing Articles

Identification of adenovirus 2 early genes required for induction of cellular DNA synthesis in resting hamster cells.

Rossini M, Jonak G, Baserga R J Virol. 1981; 38(3):982-6.

PMID: 7241670 PMC: 171237. DOI: 10.1128/JVI.38.3.982-986.1981.

Induction of cellular deoxyribonucleic acid synthesis in butyrate-treated cells by simian virus 40 deoxyribonucleic acid.

Kawasaki S, Diamond L, Baserga R Mol Cell Biol. 1981; 1(11):1038-47.

PMID: 6180295 PMC: 369725. DOI: 10.1128/mcb.1.11.1038-1047.1981.

Regulation of specific genes during the cell cycle. Utilization of homologous cDNAs and cloned sequences for studying histone gene expression in human cells.

Stein G, Stein J, Marashi F, Parker M, Sierra L Cell Biophys. 1980; 2(4):291-314.

PMID: 6163542 DOI: 10.1007/BF02785095.

Biochemistry of the cell cycle.

Lloyd D Biochem J. 1987; 242(2):313-21.

PMID: 3297045 PMC: 1147707. DOI: 10.1042/bj2420313.

The release of growth arrest by microinjection of adenovirus E1A DNA.

Stabel S, Argos P, Philipson L EMBO J. 1985; 4(9):2329-36.

PMID: 3000772 PMC: 554505. DOI: 10.1002/j.1460-2075.1985.tb03934.x.

References

DULBECCO R, Hartwell L, Vogt M . INDUCTION OF CELLULAR DNA SYNTHESIS BY POLYOMA VIRUS. Proc Natl Acad Sci U S A. 1965; 53:403-10. PMC: 219527. DOI: 10.1073/pnas.53.2.403. View

Green M, Pina M . BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION, VI. PROPERTIES OF HIGHLY PURIFIED TUMORIGENIC HUMAN ADENOVIRUSES AND THEIR DNA. Proc Natl Acad Sci U S A. 1964; 51:1251-9. PMC: 300245. DOI: 10.1073/pnas.51.6.1251. View

Pope J, Rowe W . DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE. J Exp Med. 1964; 120:121-8. PMC: 2137732. DOI: 10.1084/jem.120.2.121. View

Schlegel R, BENJAMIN T . Cellular alterations dependent upon the polyoma virus Hr-t function: separation of mitogenic from transforming capacities. Cell. 1978; 14(3):587-99. DOI: 10.1016/0092-8674(78)90244-1. View

Tjian R, Fey G, Graessmann A . Biological activity of purified simian virus 40 T antigen proteins. Proc Natl Acad Sci U S A. 1978; 75(3):1279-83. PMC: 411454. DOI: 10.1073/pnas.75.3.1279. View

Scott W, Brockman W, Nathans D . Biological activities of deletion mutants of simian virus 40. Virology. 1976; 75(2):319-34. DOI: 10.1016/0042-6822(76)90031-3. View

Pardee A, Dubrow R, Hamlin J, Kletzien R . Animal cell cycle. Annu Rev Biochem. 1978; 47:715-50. DOI: 10.1146/annurev.bi.47.070178.003435. View

Floros J, Ashihara T, Baserga R . Characterization of ts13 cells a temperature-sensitive mutant of the G1 phase of the cell cycle. Cell Biol Int Rep. 1978; 2(3):259-69. DOI: 10.1016/0309-1651(78)90006-1. View

Ashihara T, Chang S, Baserga R . Constancy of the shift-up point in two temperature-sensitive mammalian cell lines that arrest in G1. J Cell Physiol. 1978; 96(1):15-22. DOI: 10.1002/jcp.1040960103. View

10.

. Nature of temperature-sensitive mutants. J Cell Physiol. 1978; 95(3):365-6. View

11.

Rossini M, Baserga R . RNA synthesis in a cell cycle-specific temperature sensitive mutant from a hamster cell line. Biochemistry. 1978; 17(5):858-63. DOI: 10.1021/bi00598a017. View

12.

Berk A, Sharp P . Ultraviolet mapping of the adenovirus 2 early promoters. Cell. 1977; 12(1):45-55. DOI: 10.1016/0092-8674(77)90184-2. View

13.

Talavera A, Basilico C . Temperature sensitive mutants of BHK cells affected in cell cycle progression. J Cell Physiol. 1977; 92(3):425-36. DOI: 10.1002/jcp.1040920310. View

14.

Nishimoto T, Okubo C, Raskas H . Abortive replication of adenovirus 2 in the temperature-sensitive hamster cell mutant tsAF8. Virology. 1977; 78(1):1-10. DOI: 10.1016/0042-6822(77)90074-5. View

15.

Levinson A, Levine A . The isolation and identification of the adenovirus group C tumor antigens. Virology. 1977; 76(1):1-11. DOI: 10.1016/0042-6822(77)90275-6. View

16.

DUBBS D, Kit S . Reactivation of chick erythrocyte nuclei in heterokaryons with temperature-sensitive Chinese hamster cells. Somatic Cell Genet. 1976; 2(1):11-9. DOI: 10.1007/BF01539238. View

17.

Laughlin C, STROHL W . Factors regulating cellular DNA synthesis induced by adenovirus infection. II. The effects of actinomycin D on productive virus-cell systems. Virology. 1976; 74(1):44-56. DOI: 10.1016/0042-6822(76)90126-4. View

18.

Laughlin C, STROHL W . Factors regulating cellular DNA synthesis induced by adenovirus infection. I. The effects of actinomycin D on G1-arrested BHK21 cells abortively infected with type 12 adenovirus of stimulated by serum. Virology. 1976; 74(1):30-43. DOI: 10.1016/0042-6822(76)90125-2. View

19.

Philipson L, LONBERG-HOLM K, Pettersson U . Virus-receptor interaction in an adenovirus system. J Virol. 1968; 2(10):1064-75. PMC: 375437. DOI: 10.1128/JVI.2.10.1064-1075.1968. View

20.

Zimmerman Jr J, RASKA Jr K . Inhibition of adenovirus type 12 induced DNA synthesis in G1-arrested BHK21 cells by dibutyryl adenosine cyclic 3':5'-monophosphate. Nat New Biol. 1972; 239(92):145-7. DOI: 10.1038/newbio239145a0. View