Identification of Adenovirus 2 Early Genes Required for Induction of Cellular DNA Synthesis in Resting Hamster Cells

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1981 Jun 1

PMID 7241670

Citations 6

Authors

M Rossini

G J Jonak

R Baserga

Affiliations

Soon will be listed here.

Abstract

tsAF8 cells are temperature-sensitive (ts) mutants of BHK-21 cells that arrest at the nonpermissive temperature in the G1 phase of the cell cycle. When made quiescent by serum restriction, they can be stimulated to enter the S phase by 10% serum at 34 degrees C, but not at 40.6 degrees C. Infection by adenovirus type 2 or type 5 stimulates cellular DNA synthesis in tsAF8 cells at both 34 and 40.6 degrees C. Infection of these cells with deletion Ad5dl312, Ad5dl313, Ad2 delta p305, and Ad2+D1) and temperature-sensitive (H5ts125, H5ts36) mutants of adenovirus indicates that the expression of both early regions 1A and 2 is needed to induce quiescent tsAF8 cells to enter the S phase at the permissive temperature. This finding has been confirmed by microinjection of selected adenovirus DNA fragments into the nucleus of tsAF8 cells. In addition, we have shown that additional viral functions encoded by early regions 1B and 5 are required for the induction of cellular DNA synthesis at the nonpermissive temperature.

Citing Articles

Adenovirus-induced alterations of the cell growth cycle: effects of mutations in early regions E2A and E2B.

Murray J, Braithwaite A, TAYLOR I, BELLETT A J Virol. 1982; 44(3):1072-5.

PMID: 7176017 PMC: 256368. DOI: 10.1128/JVI.44.3.1072-1075.1982.

Adenovirus-induced alterations of the cell growth cycle: a requirement for expression of E1A but not of E1B.

Braithwaite A, Cheetham B, Li P, Parish C, BELLETT A J Virol. 1983; 45(1):192-9.

PMID: 6823012 PMC: 256401. DOI: 10.1128/JVI.45.1.192-199.1983.

Sequence of the junction in adenovirus 2-SV40 hybrids: examples of illegitimate recombination.

Ling L, Manos M, Gluzman Y Nucleic Acids Res. 1982; 10(24):8099-112.

PMID: 6298708 PMC: 327072. DOI: 10.1093/nar/10.24.8099.

Microinjection of monoclonal antibody to protein p53 inhibits serum-induced DNA synthesis in 3T3 cells.

Mercer W, Nelson D, DeLeo A, Old L, Baserga R Proc Natl Acad Sci U S A. 1982; 79(20):6309-12.

PMID: 6292898 PMC: 347110. DOI: 10.1073/pnas.79.20.6309.

The release of growth arrest by microinjection of adenovirus E1A DNA.

Stabel S, Argos P, Philipson L EMBO J. 1985; 4(9):2329-36.

PMID: 3000772 PMC: 554505. DOI: 10.1002/j.1460-2075.1985.tb03934.x.

References

Philipson L, LONBERG-HOLM K, Pettersson U . Virus-receptor interaction in an adenovirus system. J Virol. 1968; 2(10):1064-75. PMC: 375437. DOI: 10.1128/JVI.2.10.1064-1075.1968. View

DULBECCO R, Hartwell L, Vogt M . INDUCTION OF CELLULAR DNA SYNTHESIS BY POLYOMA VIRUS. Proc Natl Acad Sci U S A. 1965; 53:403-10. PMC: 219527. DOI: 10.1073/pnas.53.2.403. View

Pettersson U, Sambrook J . Amount of viral DNA in the genome of cells transformed by adenovirus type 2. J Mol Biol. 1973; 73(1):125-30. DOI: 10.1016/0022-2836(73)90164-2. View

Meiss H, Basilico C . Temperature sensitive mutants of BHK 21 cells. Nat New Biol. 1972; 239(90):66-8. DOI: 10.1038/newbio239066a0. View

Zimmerman Jr J, RASKA Jr K . Inhibition of adenovirus type 12 induced DNA synthesis in G1-arrested BHK21 cells by dibutyryl adenosine cyclic 3':5'-monophosphate. Nat New Biol. 1972; 239(92):145-7. DOI: 10.1038/newbio239145a0. View

Schnipper L, Lewis Jr A, Levine A . Induction of cellular DNA synthesis by the nondefective Adenovirus 2-Simian virus 40 hybrid viruses. J Virol. 1973; 12(4):940-3. PMC: 356714. DOI: 10.1128/JVI.12.4.940-943.1973. View

Burstin S, Meiss H, Basilico C . A temperature-sensitive cell cycle mutant of the BHK cell line. J Cell Physiol. 1974; 84(3):397-408. DOI: 10.1002/jcp.1040840308. View

van der Vliet P, Levine A, Ensinger M, Ginsberg H . Thermolabile DNA binding proteins from cells infected with a temperature-sensitive mutant of adenovrius defective in viral DNA synthesis. J Virol. 1975; 15(2):348-54. PMC: 354459. DOI: 10.1128/JVI.15.2.348-354.1975. View

Tanaka T, Weisblum B . Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J Bacteriol. 1975; 121(1):354-62. PMC: 285650. DOI: 10.1128/jb.121.1.354-362.1975. View

10.

Nishimoto T, Raskas H, Basilico C . Temperature-sensitive cell mutations that inhibit adenovirus 2 replication. Proc Natl Acad Sci U S A. 1975; 72(1):328-32. PMC: 432298. DOI: 10.1073/pnas.72.1.328. View

11.

Weil R, Salomon E, May E, May P . A simplifying concept in tumor virology: virus-specific "pleiotropic effectors". Cold Spring Harb Symp Quant Biol. 1975; 39 Pt 1:381-95. DOI: 10.1101/sqb.1974.039.01.050. View

12.

Sauer G, Defendi V . Stimulation of DNA synthesis and complement-fixing antigen production by SV40 in human diploid cell cultures: evidence for "abortive" infection. Proc Natl Acad Sci U S A. 1966; 56(2):452-7. PMC: 224393. DOI: 10.1073/pnas.56.2.452. View

13.

McDonell M, Simon M, Studier F . Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977; 110(1):119-46. DOI: 10.1016/s0022-2836(77)80102-2. View

14.

Grodzicker T, Anderson C, Sambrook J, Mathews M . The physical locations of structural genes in adenovirus DNA. Virology. 1977; 80(1):111-26. DOI: 10.1016/0042-6822(77)90384-1. View

15.

Rossini M, Baserga R . RNA synthesis in a cell cycle-specific temperature sensitive mutant from a hamster cell line. Biochemistry. 1978; 17(5):858-63. DOI: 10.1021/bi00598a017. View

16.

Hassell J, Lukanidin E, Fey G, Sambrook J . The structure and expression of two defective adenovirus 2/simian virus 40 hybrids. J Mol Biol. 1978; 120(2):209-47. DOI: 10.1016/0022-2836(78)90065-7. View

17.

Horwitz M . Temperature-sensitive replication of H5ts125 adenovirus DNA in vitro. Proc Natl Acad Sci U S A. 1978; 75(9):4291-5. PMC: 336099. DOI: 10.1073/pnas.75.9.4291. View

18.

Frost E, Williams J . Mapping temperature-sensitive and host-range mutations of adenovirus type 5 by marker rescue. Virology. 1978; 91(1):39-50. DOI: 10.1016/0042-6822(78)90353-7. View

19.

Jones N, Shenk T . Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells. Cell. 1979; 17(3):683-9. DOI: 10.1016/0092-8674(79)90275-7. View

20.

Berk A, Lee F, Harrison T, Williams J, Sharp P . Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs. Cell. 1979; 17(4):935-44. DOI: 10.1016/0092-8674(79)90333-7. View