Comparison of Fast-track Diagnostics Respiratory Pathogens Multiplex Real-time RT-PCR Assay with In-house Singleplex Assays for Comprehensive Detection of Human Respiratory Viruses

Overview

Journal J Virol Methods

Specialty Microbiology

Date 2012 Jul 17

PMID 22796035

Citations 52

Authors

Senthilkumar K Sakthivel

Brett Whitaker

Xiaoyan Lu

Danielle B L Oliveira

Lauren J Stockman

Shifaq Kamili

M Steven Oberste

Dean D Erdman

Affiliations

Soon will be listed here.

Abstract

Fast-track Diagnostics respiratory pathogens (FTDRP) multiplex real-time RT-PCR assay was compared with in-house singleplex real-time RT-PCR assays for detection of 16 common respiratory viruses. The FTDRP assay correctly identified 26 diverse respiratory virus strains, 35 of 41 (85%) external quality assessment samples spiked with cultured virus and 232 of 263 (88%) archived respiratory specimens that tested positive for respiratory viruses by in-house assays. Of 308 prospectively tested respiratory specimens selected from children hospitalized with acute respiratory illness, 270 (87.7%) and 265 (86%) were positive by FTDRP and in-house assays for one or more viruses, respectively, with combined test results showing good concordance (K=0.812, 95% CI=0.786-0.838). Individual FTDRP assays for adenovirus, respiratory syncytial virus and rhinovirus showed the lowest comparative sensitivities with in-house assays, with most discrepancies occurring with specimens containing low virus loads and failed to detect some rhinovirus strains, even when abundant. The FTDRP enterovirus and human bocavirus assays appeared to be more sensitive than the in-house assays with some specimens. With the exceptions noted above, most FTDRP assays performed comparably with in-house assays for most viruses while offering enhanced throughput and easy integration by laboratories using conventional real-time PCR instrumentation.

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