Bone Marrow-derived Fibroblast Growth Factor-2 Induces Glial Cell Proliferation in the Regenerating Peripheral Nervous System

Overview

Journal Mol Neurodegener

Publisher Biomed Central

Specialties Molecular Biology
Neurology

Date 2012 Jul 17

PMID 22793996

Citations 13

Authors

Victor Tulio Ribeiro-Resende

Alvaro Carrier-Ruiz

Robertha M R Lemes

Ricardo A M Reis

Rosalia Mendez-Otero

Affiliations

Soon will be listed here.

Abstract

Background: Among the essential biological roles of bone marrow-derived cells, secretion of many soluble factors is included and these small molecules can act upon specific receptors present in many tissues including the nervous system. Some of the released molecules can induce proliferation of Schwann cells (SC), satellite cells and lumbar spinal cord astrocytes during early steps of regeneration in a rat model of sciatic nerve transection. These are the major glial cell types that support neuronal survival and axonal growth following peripheral nerve injury. Fibroblast growth factor-2 (FGF-2) is the main mitogenic factor for SCs and is released in large amounts by bone marrow-derived cells, as well as by growing axons and endoneurial fibroblasts during development and regeneration of the peripheral nervous system (PNS).

Results: Here we show that bone marrow-derived cell treatment induce an increase in the expression of FGF-2 in the sciatic nerve, dorsal root ganglia and the dorsolateral (DL) region of the lumbar spinal cord (LSC) in a model of sciatic nerve transection and connection into a hollow tube. SCs in culture in the presence of bone marrow derived conditioned media (CM) resulted in increased proliferation and migration. This effect was reduced when FGF-2 was neutralized by pretreating BMMC or CM with a specific antibody. The increased expression of FGF-2 was validated by RT-PCR and immunocytochemistry in co-cultures of bone marrow derived cells with sciatic nerve explants and regenerating nerve tissue respectivelly.

Conclusion: We conclude that FGF-2 secreted by BMMC strongly increases early glial proliferation, which can potentially improve PNS regeneration.

Citing Articles

All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve Regeneration.

Pinero G, Vence M, Aranda M, Cercato M, Soto P, Usach V ASN Neuro. 2023; 15:17590914231167281.

PMID: 37654230 PMC: 10475269. DOI: 10.1177/17590914231167281.

Sequential expression of miR-221-3p and miR-338-3p in Schwann cells as a therapeutic strategy to promote nerve regeneration and functional recovery.

Wen L, Yu T, Ma Y, Mao X, Ao T, Javed R Neural Regen Res. 2022; 18(3):671-682.

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Progression in translational research on spinal cord injury based on microenvironment imbalance.

Fan B, Wei Z, Feng S Bone Res. 2022; 10(1):35.

PMID: 35396505 PMC: 8993811. DOI: 10.1038/s41413-022-00199-9.

Stem Cells and Tissue Engineering-Based Therapeutic Interventions: Promising Strategies to Improve Peripheral Nerve Regeneration.

de Assis A, Reis A, Nunes L, Ferreira L, Bilal M, Iqbal H Cell Mol Neurobiol. 2022; 43(2):433-454.

PMID: 35107689 PMC: 11415205. DOI: 10.1007/s10571-022-01199-3.

Application of neurotrophic and proangiogenic factors as therapy after peripheral nervous system injury.

Idrisova K, Zeinalova A, Masgutova G, Bogov A, Allegrucci C, Syromiatnikova V Neural Regen Res. 2021; 17(6):1240-1247.

PMID: 34782557 PMC: 8643040. DOI: 10.4103/1673-5374.327329.

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