Fibrillized Peptide Microgels for Cell Encapsulation and 3D Cell Culture

Overview

Journal Soft Matter

Publisher Royal Society of Chemistry

Specialties Biochemistry
Chemistry

Date 2012 Jul 10

PMID 22773926

Citations 19

Authors

Ye F Tian

Jason M Devgun

Joel H Collier

Affiliations

Soon will be listed here.

Abstract

One of the advantages of materials produced by self-assembly is that in principle they can be formed in any given container to produce materials of predetermined shapes and sizes. Here, we developed a method for triggering peptide self-assembly within the aqueous phase of water-in-oil emulsions to produce spherical microgels composed of fibrillized peptides. Size control over the microgels was achieved by specification of blade type, speed, and additional shear steps in the emulsion process. Microgels constructed in this way could then be embedded within other self-assembled peptide matrices by mixing pre-formed microgels with un-assembled peptides and inducing gelation of the entire composite, offering a route towards multi-peptide materials with micron-scale domains of different peptide formulations. The gels themselves were cytocompatible, as was the microgel fabrication procedure, enabling the encapsulation of NIH 3T3 fibroblasts and C3H10T-1/2 mouse pluripotent stem cells with good viability.

Citing Articles

Localized Enzyme-Assisted Self-Assembly in the Presence of Hyaluronic Acid for Hybrid Supramolecular Hydrogel Coating.

Fores J, Bigo-Simon A, Wagner D, Payrastre M, Damestoy C, Blandin L Polymers (Basel). 2021; 13(11).

PMID: 34072331 PMC: 8198348. DOI: 10.3390/polym13111793.

Engineered Microgels-Their Manufacturing and Biomedical Applications.

Alzanbaki H, Moretti M, Hauser C Micromachines (Basel). 2021; 12(1).

PMID: 33401474 PMC: 7824414. DOI: 10.3390/mi12010045.

Natural and Synthetic Biomaterials for Engineering Multicellular Tumor Spheroids.

Kamatar A, Gunay G, Acar H Polymers (Basel). 2020; 12(11).

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Self-Assembling Behavior of pH-Responsive Peptide AK without End-Capping.

Zhang P, Wang F, Wang Y, Li S, Wen S Molecules. 2020; 25(9).

PMID: 32357459 PMC: 7249138. DOI: 10.3390/molecules25092017.

Peptide-Based Physical Gels Endowed with Thixotropic Behaviour.

Zanna N, Tomasini C Gels. 2019; 3(4).

PMID: 30920535 PMC: 6318593. DOI: 10.3390/gels3040039.

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