Prophylactic Zinc Supplementation Reduces Bacterial Load and Improves Survival in a Murine Model of Sepsis

Overview

Journal Pediatr Crit Care Med

Specialty Pediatrics

Date 2012 Jul 5

PMID 22760431

Citations 27

Authors

Jeffrey E Nowak

Kelli Harmon

Charles C Caldwell

Hector R Wong

Affiliations

Soon will be listed here.

Abstract

Objective: We previously demonstrated that altered zinc homeostasis is an important feature of pediatric sepsis, thus raising the possibility of zinc supplementation as a therapeutic strategy in sepsis. Herein, we tested the hypothesis that prophylactic zinc supplementation would be beneficial in a murine model of peritoneal sepsis.

Design: Murine model of sepsis (intraperitoneal fecal-slurry injection).

Setting: Basic science research laboratory.

Subjects: C57BL/6 male mice.

Interventions: Intraperitoneal fecal-slurry injection, with or without zinc supplementation (10 mg/kg of intraperitoneal zinc gluconate for 3 days prior to intraperitoneal fecal-slurry injection).

Measurements And Main Results: Survival over 3 days following intraperitoneal fecal-slurry injection, markers of inflammation, bacterial load studies, and immunophenotyping studies. Zinc-supplemented mice demonstrated a significant survival advantage compared to control (nonsupplemented) mice. Zinc-supplemented mice also demonstrated moderate reductions of inflammation and immune activation. The survival advantage primarily correlated with reduced in vivo bacterial load in zinc-supplemented mice, compared to controls. In addition, peritoneal macrophages harvested from zinc-supplemented mice demonstrated a significantly enhanced phagocytosis capacity for Escherichia coli and Staphylococcus aureus, compared to peritoneal macrophages harvested from control mice.

Conclusion: Prophylactic zinc supplementation reduces bacterial load and is beneficial in a murine model of peritoneal sepsis.

Citing Articles

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