Genetic Polymorphisms Influence the Ovarian Response to RFSH Stimulation in Patients Undergoing in Vitro Fertilization Programs with ICSI

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Jun 16

PMID 22701696

Citations 36

Authors

Radia Boudjenah

Denise Molina-Gomes

Antoine Torre

Marianne Bergere

Marc Bailly

Florence Boitrelle

Stephane Taieb

Robert Wainer

Mohamed Benahmed

Philippe de Mazancourt

Jacqueline Selva

Francois Vialard

Affiliations

Soon will be listed here.

Abstract

Introduction: Obtaining an adequate number of high-quality oocytes is a major challenge in controlled ovarian hyperstimulation (COH). To date, a range of hormonal and clinical parameters have been used to optimize COH but none have significant predictive value. This variability could be due to the genetic predispositions of single-nucleotide polymorphisms (SNPs). Here, we assessed the individual and combined impacts of thirteen SNPs that reportedly influence the outcome of in vitro fertilisation (IVF) on the ovarian response to rFSH stimulation for patients undergoing intracytoplasmic sperm injection program (ICSI).

Results: Univariate analysis revealed that only FSHR, ESR2 and p53 SNPs influenced the number of mature oocytes. The association was statistically significant for FSHR (p=0.0047) and ESR2 (0.0017) in the overall study population and for FSHR (p=0.0009) and p53 (p=0.0048) in subgroup that was more homogeneous in terms of clinical variables. After Bonferroni correction and a multivariate analysis, only the differences for FSHR and ESR2 polymorphisms were still statistically significant. In a multilocus analysis, only the FSHR and AMH SNP combination significantly influenced oocyte numbers in both population (p<0.01).

Discussion: We confirmed the impact of FSHR and ESR2 polymorphisms on the IVF outcome. Furthermore, we showed for the first time that a p53 polymorphism (which is already known to impact embryo implantation) could influence the ovarian response. However, given that this result lost its statistical significance after multivariate analysis, more data are needed to draw firm conclusions. Only the FSHR and AMH polymorphism combination appears to influence mature oocyte numbers but this finding also needs to be confirmed.

Materials And Methods: A 13 gene polymorphisms: FSHR(Asn680Ser), p53(Arg72Pro), AMH(Ile49Ser), ESR2(+1730G>A), ESR1(-397T>C), BMP15(-9C>G), MTHFR1(677C>T), MTHFR2(1298A>C), HLA-G(-725C>G), VEGF(+405G>C), TNFα(-308A>G), AMHR(-482 A>G), PAI-1 (4 G/5 G), multiplex PCR assay was designed to genotype women undergoing ICSI program. We analyzed the overall study population (n=427) and a subgroup with homogeneous characteristics (n=112).

Citing Articles

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Population Pharmacokinetic Analysis of Follicle-Stimulating Hormone During Ovarian Stimulation: Relation with Weight, Prolactin and Gene Polymorphism in THADA and ADIPOQ.

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PMID: 37632631 DOI: 10.1007/s40262-023-01299-3.

The Ala307Thr polymorphism of the follicle-stimulating hormone receptor (FSHR) gene is associated with the dose of recombinant FSH received during IVF/ICSI treatment.

Dieamant F, Petersen C, Vagnini L, Petersen B, Ricci J, Nicoletti A JBRA Assist Reprod. 2023; 27(1):78-84.

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The Polymorphism Asn680Ser on the FSH Receptor and Abnormal Ovarian Response in Patients with Normal Values of AMH and AFC.

Baldini G, Catino A, Palini S, Sciorio R, Ferri D, Vinciguerra M Int J Mol Sci. 2023; 24(2).

PMID: 36674596 PMC: 9865618. DOI: 10.3390/ijms24021080.

The Effect of the Allelics of Ser680Asn Polymorphisms of Follicle-Stimulating Hormone Receptor Gene in IVF/ICSI Cycles: a Systematic Review and Meta-analysis.

Prodromidou A, Dimitroulia E, Mavrogianni D, Kathopoulis N, Pappa K, Loutradis D Reprod Sci. 2022; 30(2):428-441.

PMID: 35680725 DOI: 10.1007/s43032-022-00996-x.

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