Genome, Integration, and Transduction of a Novel Temperate Phage of Helicobacter Pylori

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2012 Jun 15

PMID 22696647

Citations 27

Authors

Cheng-Hung Luo

Pei-Yu Chiou

Chiou-Ying Yang

Nien-Tsung Lin

Affiliations

Soon will be listed here.

Abstract

Helicobacter pylori is a common human pathogen that has been identified to be carcinogenic. This study isolated the temperate bacteriophage 1961P from the lysate of a clinical strain of H. pylori isolated in Taiwan. The bacteriophage has an icosahedral head and a short tail, typical of the Podoviridae family. Its double-stranded DNA genome is 26,836 bp long and has 33 open reading frames. Only 9 of the predicted proteins have homologs of known functions, while the remaining 24 are only similar to unknown proteins encoded by Helicobacter prophages and remnants. Analysis of sequences proximal to the phage-host junctions suggests that 1961P may integrate into the host chromosome via a mechanism similar to that of bacteriophage lambda. In addition, 1961P is capable of generalized transduction. To the best of our knowledge, this is the first report of the isolation, characterization, genome analysis, integration, and transduction of a Helicobacter pylori phage.

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References

Lehours P, Vale F, Bjursell M, Melefors O, Advani R, Glavas S . Genome sequencing reveals a phage in Helicobacter pylori. mBio. 2011; 2(6). PMC: 3221604. DOI: 10.1128/mBio.00239-11. View

De Francesco V, Giorgio F, Hassan C, Manes G, Vannella L, Panella C . Worldwide H. pylori antibiotic resistance: a systematic review. J Gastrointestin Liver Dis. 2010; 19(4):409-14. View

Heintschel von Heinegg E, Nalik H, Schmid E . Characterisation of a Helicobacter pylori phage (HP1). J Med Microbiol. 1993; 38(4):245-9. DOI: 10.1099/00222615-38-4-245. View

Lin N, Chiou P, Chang K, Chen L, Lai M . Isolation and characterization of phi AB2: a novel bacteriophage of Acinetobacter baumannii. Res Microbiol. 2010; 161(4):308-14. DOI: 10.1016/j.resmic.2010.03.007. View

Bowman B, Newman H, Moritz J, Koehler R . Properties of mycobacteriophage DS6A. II. Lipid composition. Am Rev Respir Dis. 1973; 107(1):42-9. DOI: 10.1164/arrd.1973.107.1.42. View

van Vliet A, Wooldridge K, Ketley J . Iron-responsive gene regulation in a campylobacter jejuni fur mutant. J Bacteriol. 1998; 180(20):5291-8. PMC: 107575. DOI: 10.1128/JB.180.20.5291-5298.1998. View

McCloy E . Lysogenicity and immunity to Bacillus phage W. J Gen Microbiol. 1958; 18(1):198-220. DOI: 10.1099/00221287-18-1-198. View

Schmid E, von Recklinghausen G, Ansorg R . Bacteriophages in Helicobacter (Campylobacter) pylori. J Med Microbiol. 1990; 32(2):101-4. DOI: 10.1099/00222615-32-2-101. View

Josenhans C, Labigne A, Suerbaum S . Comparative ultrastructural and functional studies of Helicobacter pylori and Helicobacter mustelae flagellin mutants: both flagellin subunits, FlaA and FlaB, are necessary for full motility in Helicobacter species. J Bacteriol. 1995; 177(11):3010-20. PMC: 176987. DOI: 10.1128/jb.177.11.3010-3020.1995. View

10.

Rao V, Feiss M . The bacteriophage DNA packaging motor. Annu Rev Genet. 2008; 42:647-81. DOI: 10.1146/annurev.genet.42.110807.091545. View

11.

Berk R, GRONKOWSKI L . PROPERTIES OF PSEUDOMONAS AERUGINOSA EXHIBITING SELF-LYSIS. Antonie Van Leeuwenhoek. 1964; 30:141-53. DOI: 10.1007/BF02046720. View

12.

Tanner N, Cordin O, Banroques J, Doere M, Linder P . The Q motif: a newly identified motif in DEAD box helicases may regulate ATP binding and hydrolysis. Mol Cell. 2003; 11(1):127-38. DOI: 10.1016/s1097-2765(03)00006-6. View

13.

Akada J, Shirai M, Takeuchi H, Tsuda M, Nakazawa T . Identification of the urease operon in Helicobacter pylori and its control by mRNA decay in response to pH. Mol Microbiol. 2000; 36(5):1071-84. DOI: 10.1046/j.1365-2958.2000.01918.x. View

14.

Uemura N, Okamoto S, Yamamoto S, Matsumura N, Yamaguchi S, Yamakido M . Helicobacter pylori infection and the development of gastric cancer. N Engl J Med. 2001; 345(11):784-9. DOI: 10.1056/NEJMoa001999. View

15.

Hofreuter D, Odenbreit S, Haas R . Natural transformation competence in Helicobacter pylori is mediated by the basic components of a type IV secretion system. Mol Microbiol. 2001; 41(2):379-91. DOI: 10.1046/j.1365-2958.2001.02502.x. View

16.

Sternberg N, Coulby J . Recognition and cleavage of the bacteriophage P1 packaging site (pac). II. Functional limits of pac and location of pac cleavage termini. J Mol Biol. 1987; 194(3):469-79. DOI: 10.1016/0022-2836(87)90675-9. View

17.

Wan X, Tang D, Liu A, Tan S, Li W, Kuang J . [Isolation of a wild-type virulent phage of Helicobacter pylori and its simulated treatments of gastrointestinal Hp in vitro]. Nan Fang Yi Ke Da Xue Xue Bao. 2011; 31(2):304-7. View

18.

Rashel M, Uchiyama J, Takemura I, Hoshiba H, Ujihara T, Takatsuji H . Tail-associated structural protein gp61 of Staphylococcus aureus phage phi MR11 has bifunctional lytic activity. FEMS Microbiol Lett. 2008; 284(1):9-16. DOI: 10.1111/j.1574-6968.2008.01152.x. View

19.

Peek Jr R, Blaser M . Helicobacter pylori and gastrointestinal tract adenocarcinomas. Nat Rev Cancer. 2002; 2(1):28-37. DOI: 10.1038/nrc703. View

20.

Hu C, Wu C, Lin C, Cheng C, Su S, Tseng Y . Resistance rate to antibiotics of Helicobacter pylori isolates in eastern Taiwan. J Gastroenterol Hepatol. 2007; 22(5):720-3. DOI: 10.1111/j.1440-1746.2006.04743.x. View