Effect of AGM and Fetal Liver-derived Stromal Cell Lines on Globin Expression in Adult Baboon (P. Anubis) Bone Marrow-derived Erythroid Progenitors

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Jun 14

PMID 22693559

Citations 4

Authors

Donald Lavelle

Kestutis Vaitkus

Maria Armila Ruiz

Vinzon Ibanez

Tatiana Kouznetsova

Yogen Saunthararajah

Nadim Mahmud

Joseph DeSimone

Affiliations

Soon will be listed here.

Abstract

This study was performed to investigate the hypothesis that the erythroid micro-environment plays a role in regulation of globin gene expression during adult erythroid differentiation. Adult baboon bone marrow and human cord blood CD34+ progenitors were grown in methylcellulose, liquid media, and in co-culture with stromal cell lines derived from different developmental stages in identical media supporting erythroid differentiation to examine the effect of the micro-environment on globin gene expression. Adult progenitors express high levels of γ-globin in liquid and methylcellulose media but low, physiological levels in stromal cell co-cultures. In contrast, γ-globin expression remained high in cord blood progenitors in stromal cell line co-cultures. Differences in γ-globin gene expression between adult progenitors in stromal cell line co-cultures and liquid media required cell-cell contact and were associated with differences in rate of differentiation and γ-globin promoter DNA methylation. We conclude that γ-globin expression in adult-derived erythroid cells can be influenced by the micro-environment, suggesting new potential targets for HbF induction.

Citing Articles

Epigenetic regulation of hemoglobin switching in non-human primates.

Molokie R, DeSimone J, Lavelle D Semin Hematol. 2021; 58(1):10-14.

PMID: 33509438 PMC: 7876624. DOI: 10.1053/j.seminhematol.2020.12.001.

Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1.

Grieco A, Billett H, Green N, Driscoll M, Bouhassira E PLoS One. 2015; 10(6):e0129431.

PMID: 26053062 PMC: 4459969. DOI: 10.1371/journal.pone.0129431.

Hydroxymethylcytosine and demethylation of the γ-globin gene promoter during erythroid differentiation.

Ruiz M, Rivers A, Ibanez V, Vaitkus K, Mahmud N, DeSimone J Epigenetics. 2015; 10(5):397-407.

PMID: 25932923 PMC: 4622718. DOI: 10.1080/15592294.2015.1039220.

RN-1, a potent and selective lysine-specific demethylase 1 inhibitor, increases γ-globin expression, F reticulocytes, and F cells in a sickle cell disease mouse model.

Rivers A, Vaitkus K, Ruiz M, Ibanez V, Jagadeeswaran R, Kouznetsova T Exp Hematol. 2015; 43(7):546-53.e1-3.

PMID: 25931013 PMC: 6325640. DOI: 10.1016/j.exphem.2015.04.005.

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