Influence of Mitochondrial DNA Level on Cellular Energy Metabolism: Implications for Mitochondrial Diseases

Overview

Journal J Bioenerg Biomembr

Publisher Springer

Specialties Biochemistry
Biology
Endocrinology

Date 2008 Apr 17

PMID 18415670

Citations 23

Authors

Christophe Rocher

Jan-Willem Taanman

Denis Pierron

Benjamin Faustin

Giovani Benard

Rodrigue Rossignol

Monique Malgat

Laurence Pedespan

Thierry Letellier

Affiliations

Soon will be listed here.

Abstract

The total amount of cellular mitochondrial DNA (mtDNA) varies widely and seems to be related to the nature and metabolic state of tissues and cells in culture. It is not known, however, whether this variation has any significance in vivo, and to which extent it regulates energy production. To better understand the importance of the cellular mtDNA level, we studied the influence of a gradual reduction of mtDNA copy number on oxidative phosphorylation in two models: (a) a control human cell line treated with different concentrations of 2', 3'-dideoxycytidine, a nucleoside analogue that inhibits mtDNA replication by interfering with mitochondrial DNA polymerase gamma, and (b) a cell line derived from a patient presenting mtDNA depletion. The two models were used to construct biochemical and phenotypic threshold curves. Our results show that oxidative phosphorylation activities are under a tight control by the amount of mtDNA in the cell, and that the full complement of mtDNA molecules are necessary to maintain a normal energy production level.

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