Molecular Interactions of GBF1 with HY5 and HYH Proteins During Light-mediated Seedling Development in Arabidopsis Thaliana

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2012 Jun 14

PMID 22692212

Citations 44

Authors

Aparna Singh

Hathi Ram

Nazia Abbas

Sudip Chattopadhyay

Affiliations

Soon will be listed here.

Abstract

Arabidopsis bZIP transcription factor, GBF1, acts as a differential regulator of cryptochrome-mediated blue light signaling. Whereas the bZIP proteins, HY5 (elongated hypocotyl 5) and HYH (HY5 homologue), are degraded by COP1-mediated proteasomal pathways, GBF1 is degraded by a proteasomal pathway independent of COP1. In this study, we have investigated the functional interrelations of GBF1 with HY5 and HYH in Arabidopsis seedling development. The genetic studies using double and triple mutants reveal that GBF1 largely acts antagonistically with HY5 and HYH in Arabidopsis seedling development. Further, GBF1 and HY5 play more important roles than HYH in blue light-mediated photomorphogenic growth. This study reveals that GBF1 is able to form a G-box-binding heterodimer with HY5 but not with HYH. The in vitro and in vivo studies demonstrate that GBF1 co-localizes with HY5 or HYH in the nucleus and physically interacts with both of the proteins. The protein-protein interaction studies further reveal that the bZIP domain of GBF1 is essential and sufficient for the interaction with HY5 or HYH. Taken together, these data demonstrate the functional interrelations of GBF1 with HY5 and HYH in Arabidopsis seedling development.

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References

Ma L, Li J, Qu L, Hager J, Chen Z, Zhao H . Light control of Arabidopsis development entails coordinated regulation of genome expression and cellular pathways. Plant Cell. 2001; 13(12):2589-607. PMC: 139475. DOI: 10.1105/tpc.010229. View

Whitelam G, Johnson E, Peng J, Carol P, Anderson M, Cowl J . Phytochrome A null mutants of Arabidopsis display a wild-type phenotype in white light. Plant Cell. 1993; 5(7):757-68. PMC: 160314. DOI: 10.1105/tpc.5.7.757. View

Harter K, Kircher S, Frohnmeyer H, Krenz M, Nagy F, Schafer E . Light-regulated modification and nuclear translocation of cytosolic G-box binding factors in parsley. Plant Cell. 1994; 6(4):545-59. PMC: 160457. DOI: 10.1105/tpc.6.4.545. View

Chattopadhyay S, Ang L, Puente P, Deng X, Wei N . Arabidopsis bZIP protein HY5 directly interacts with light-responsive promoters in mediating light control of gene expression. Plant Cell. 1998; 10(5):673-83. PMC: 144028. DOI: 10.1105/tpc.10.5.673. View

Walter M, Chaban C, Schutze K, Batistic O, Weckermann K, Nake C . Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. Plant J. 2004; 40(3):428-38. DOI: 10.1111/j.1365-313X.2004.02219.x. View

Yoo S, Cho Y, Sheen J . Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc. 2007; 2(7):1565-72. DOI: 10.1038/nprot.2007.199. View

Tepperman J, Zhu T, Chang H, Wang X, Quail P . Multiple transcription-factor genes are early targets of phytochrome A signaling. Proc Natl Acad Sci U S A. 2001; 98(16):9437-42. PMC: 55439. DOI: 10.1073/pnas.161300998. View

Siberil Y, Doireau P, Gantet P . Plant bZIP G-box binding factors. Modular structure and activation mechanisms. Eur J Biochem. 2001; 268(22):5655-66. DOI: 10.1046/j.0014-2956.2001.02552.x. View

Lee J, He K, Stolc V, Lee H, Figueroa P, Gao Y . Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development. Plant Cell. 2007; 19(3):731-49. PMC: 1867377. DOI: 10.1105/tpc.106.047688. View

10.

Bhatia S, Gangappa S, Kushwaha R, Kundu S, Chattopadhyay S . SHORT HYPOCOTYL IN WHITE LIGHT1, a serine-arginine-aspartate-rich protein in Arabidopsis, acts as a negative regulator of photomorphogenic growth. Plant Physiol. 2008; 147(1):169-78. PMC: 2330309. DOI: 10.1104/pp.108.118174. View

11.

Pepper A, Chory J . Extragenic suppressors of the Arabidopsis det1 mutant identify elements of flowering-time and light-response regulatory pathways. Genetics. 1997; 145(4):1125-37. PMC: 1207881. DOI: 10.1093/genetics/145.4.1125. View

12.

Ang L, Deng X . Regulatory hierarchy of photomorphogenic loci: allele-specific and light-dependent interaction between the HY5 and COP1 loci. Plant Cell. 1994; 6(5):613-28. PMC: 160463. DOI: 10.1105/tpc.6.5.613. View

13.

Schindler U, Menkens A, Beckmann H, Ecker J, Cashmore A . Heterodimerization between light-regulated and ubiquitously expressed Arabidopsis GBF bZIP proteins. EMBO J. 1992; 11(4):1261-73. PMC: 556574. DOI: 10.1002/j.1460-2075.1992.tb05170.x. View

14.

Mallappa C, Singh A, Ram H, Chattopadhyay S . GBF1, a transcription factor of blue light signaling in Arabidopsis, is degraded in the dark by a proteasome-mediated pathway independent of COP1 and SPA1. J Biol Chem. 2008; 283(51):35772-82. DOI: 10.1074/jbc.M803437200. View

15.

Osterlund M, Deng X . Multiple photoreceptors mediate the light-induced reduction of GUS-COP1 from Arabidopsis hypocotyl nuclei. Plant J. 1998; 16(2):201-8. DOI: 10.1046/j.1365-313x.1998.00290.x. View

16.

Schindler U, Terzaghi W, Beckmann H, Kadesch T, Cashmore A . DNA binding site preferences and transcriptional activation properties of the Arabidopsis transcription factor GBF1. EMBO J. 1992; 11(4):1275-89. PMC: 556575. DOI: 10.1002/j.1460-2075.1992.tb05171.x. View

17.

Neff M, Fankhauser C, Chory J . Light: an indicator of time and place. Genes Dev. 2000; 14(3):257-71. View

18.

Lin C . Phototropin blue light receptors and light-induced movement responses in plants. Sci STKE. 2002; 2002(118):pe5. DOI: 10.1126/stke.2002.118.pe5. View

19.

Koornneef M, van der Veen J . Induction and analysis of gibberellin sensitive mutants in Arabidopsis thaliana (L.) heynh. Theor Appl Genet. 2013; 58(6):257-63. DOI: 10.1007/BF00265176. View

20.

Saijo Y, Sullivan J, Wang H, Yang J, Shen Y, Rubio V . The COP1-SPA1 interaction defines a critical step in phytochrome A-mediated regulation of HY5 activity. Genes Dev. 2003; 17(21):2642-7. PMC: 280614. DOI: 10.1101/gad.1122903. View