Myosin 1E Coordinates Actin Assembly and Cargo Trafficking During Clathrin-mediated Endocytosis

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2012 Jun 8

PMID 22675027

Citations 63

Authors

Jackie Cheng

Alexandre Grassart

David G Drubin

Affiliations

Soon will be listed here.

Abstract

Myosin 1E (Myo1E) is recruited to sites of clathrin-mediated endocytosis coincident with a burst of actin assembly. The recruitment dynamics and lifetime of Myo1E are similar to those of tagged actin polymerization regulatory proteins. Like inhibition of actin assembly, depletion of Myo1E causes reduced transferrin endocytosis and a significant delay in transferrin trafficking to perinuclear compartments, demonstrating an integral role for Myo1E in these actin-mediated steps. Mistargeting of GFP-Myo1E or its src-homology 3 domain to mitochondria results in appearance of WIP, WIRE, N-WASP, and actin filaments at the mitochondria, providing evidence for Myo1E's role in actin assembly regulation. These results suggest for mammalian cells, similar to budding yeast, interdependence in the recruitment of type I myosins, WIP/WIRE, and N-WASP to endocytic sites for Arp2/3 complex activation to assemble F-actin as endocytic vesicles are being formed.

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